REB1 DNA-binding protein REB1 [Saccharomyces cerevisiae S288C] - Gene

Summary

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Gene symbol: REB1
Gene description: DNA-binding protein REB1
Primary source: FungiDB:YBR049C
Locus tag: YBR049C
See related: SGD:S000000253; AllianceGenome:SGD:S000000253
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Saccharomyces cerevisiae S288C (strain: S288C)
Lineage: Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces
Also known as: EBF1; GRF2
Summary: Enables sequence-specific DNA binding activity. Involved in regulation of transcription by RNA polymerase II; termination of RNA polymerase II transcription; and transcriptional start site selection at RNA polymerase II promoter. Located in nucleoplasm. Orthologous to several human genes including DMTF1 (cyclin D binding myb like transcription factor 1). [provided by Alliance of Genome Resources, Apr 2022]
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Genomic context

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See REB1 in Genome Data Viewer

Location:: chromosome: II

Exon count:: 1

Sequence:: Chromosome: II; NC_001134.8 (334386..336818, complement)

Chromosome II - NC_001134.8 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:: NC_001134.8

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Sir2 and Reb1 antagonistically regulate nucleosome occupancy in subtelomeric X-elements and repress TERRAs by distinct mechanisms.
Title: Sir2 and Reb1 antagonistically regulate nucleosome occupancy in subtelomeric X-elements and repress TERRAs by distinct mechanisms.
Reb1, Cbf1, and Pho4 Bias Histone Sliding and Deposition Away from Their Binding Sites.
Title: Reb1, Cbf1, and Pho4 Bias Histone Sliding and Deposition Away from Their Binding Sites.
Both the binding and dissociation rates of Reb1 and Cbf1 are significantly slower at the nucleosomal sites relative to those for naked DNA, demonstrating that the high affinities are achieved by increasing the dwell time on nucleosomes in order to compensate for reduced binding.
Title: Dissociation rate compensation mechanism for budding yeast pioneer transcription factors.
Abf1, Reb1, and Tbf1 control ribosome biogenesis gene expression in budding yeast.
Title: Abf1 and other general regulatory factors control ribosome biogenesis gene expression in budding yeast.
It was found that ABF1, FKH2, and REB1 overexpression decreased the growth of strains in a prion-dependent manner and, thus, might influence [PSI;(+)] prion toxicity.
Title: [Identification of new genes that affect [PSI^(+)] prion toxicity in Saccharomyces cerevisiae yeast].
Reb1p indeed binds to the TFC6 promoter, and is important for proper transcription start site selection and protein expression. Interestingly, loss of Reb1p association at this site has a similar effect on the adjacent divergently transcribed ESC2 promoter, resulting in a significant increase of 5'-extended ESC2 transcripts and reduction of Esc2 protein levels.
Title: Transcription factor Reb1 is required for proper transcriptional start site usage at the divergently transcribed TFC6-ESC2 locus in Saccharomyces cerevisiae.
Heterozygous diploid knockout strains for FHL1, RAP1, REB1, and MCM1 genes showed significantly short lifespan.
Title: Transcription factor genes essential for cell proliferation and replicative lifespan in budding yeast.
Data show that the transcriptional activator Reb1p bound to DNA is a roadblock for RNAPII, which pauses and is ubiquitinated, thus triggering termination
Title: Roadblock termination by reb1p restricts cryptic and readthrough transcription.
Report interaction of transcriptional regulators with specific nucleosomes across the Saccharomyces genome.
Title: Interaction of transcriptional regulators with specific nucleosomes across the Saccharomyces genome.

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Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Homology

Orthologs from OrthoDB

Gene Ontology Provided by SGD

Function	Evidence Code	Pubs
enables DNA binding	IEA Inferred from Electronic Annotation more info
enables DNA-binding transcription factor activity	IBA Inferred from Biological aspect of Ancestor more info
enables sequence-specific DNA binding	IDA Inferred from Direct Assay more info	PubMed
enables transcription cis-regulatory region binding	IBA Inferred from Biological aspect of Ancestor more info

Process	Evidence Code	Pubs
involved_in regulation of DNA-templated transcription	IBA Inferred from Biological aspect of Ancestor more info
involved_in regulation of transcription by RNA polymerase II	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in termination of RNA polymerase II transcription	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in transcriptional start site selection at RNA polymerase II promoter	IDA Inferred from Direct Assay more info	PubMed
involved_in transcriptional start site selection at RNA polymerase II promoter	IMP Inferred from Mutant Phenotype more info	PubMed

Component	Evidence Code	Pubs
located_in nucleoplasm	IDA Inferred from Direct Assay more info	PubMed
is_active_in nucleus	IBA Inferred from Biological aspect of Ancestor more info
located_in nucleus	IEA Inferred from Electronic Annotation more info

General protein information

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Preferred Names: DNA-binding protein REB1

NP_009605.1

RNA polymerase I enhancer binding protein; DNA binding protein that binds to genes transcribed by both RNA polymerase I and RNA polymerase II; required for termination of RNA polymerase I transcription; Reb1p bound to DNA acts to block RNA polymerase II readthrough transcription

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

NC_001134.8 Reference assembly

Range

334386..336818 complement

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GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

NM_001178397.1 → NP_009605.1 TPA: DNA-binding protein REB1 [Saccharomyces cerevisiae S288C]

See identical proteins and their annotated locations for NP_009605.1

Status: REVIEWED

UniProtKB/Swiss-Prot

D6VQ49, P21538

UniProtKB/TrEMBL

A0A8H4BU13, A6ZKZ3, B3LN99, C7GST6, G2W955, N1P8A1

Conserved Domains (2) summary

smart00717
Location:691 → 719

SANT; SANT SWI3, ADA2, N-CoR and TFIIIB'' DNA-binding domains

COG5147
Location:202 → 701

REB1; Myb superfamily proteins, including transcription factors and mRNA splicing factors [Transcription / RNA processing and modification / Cell division and chromosome partitioning]