Pfkm phosphofructokinase, muscle [Rattus norvegicus (Norway rat)] - Gene

Summary

Official Symbol: Pfkmprovided by RGD
Official Full Name: phosphofructokinase, muscleprovided by RGD
Primary source: RGD:68419
See related: Ensembl:ENSRNOG00000057988 AllianceGenome:RGD:68419
Gene type: protein coding
RefSeq status: VALIDATED
Organism: Rattus norvegicus
Lineage: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Rattus
Also known as: PFK-A; Pfk-M; ATP-PFK
Summary: Enables several functions, including 6-phosphofructokinase activity; anion binding activity; and identical protein binding activity. Involved in fructose 1,6-bisphosphate metabolic process; fructose 6-phosphate metabolic process; and glycolytic process. Predicted to be located in several cellular components, including apical plasma membrane; cytosol; and sperm principal piece. Predicted to be part of 6-phosphofructokinase complex. Predicted to be active in membrane. Human ortholog(s) of this gene implicated in glycogen storage disease VII. Orthologous to human PFKM (phosphofructokinase, muscle). [provided by Alliance of Genome Resources, Apr 2022]
Expression: Biased expression in Muscle (RPKM 4937.7), Heart (RPKM 1078.7) and 1 other tissue See more
Orthologs: human mouse all
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Genomic context

Location:: 7q36

Exon count:: 26

Annotation release	Status	Assembly	Chr	Location
RS_2024_02	current	GRCr8 (GCF_036323735.1)	7	NC_086025.1 (131100684..131138250)
RS_2023_06	previous assembly	mRatBN7.2 (GCF_015227675.2)	7	NC_051342.1 (129221679..129259192)
106	previous assembly	Rnor_6.0 (GCF_000001895.5)	7	NC_005106.4 (139702066..139722132)

Chromosome 7 - NC_086025.1 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:

Go to nucleotide: Graphics FASTA GenBank

Expression

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See details

Project title: A rat RNA-Seq transcriptomic BodyMap across 11 organs and 4 developmental stages
Description: 320 RNA samples isolated from 11 organs (adrenal gland, brain, heart, kidney, liver, lung, muscle, spleen, thymus, and testes or uterus) from both sexes of Fischer 344 rats across four developmental stages (2-, 6-, 21-, and 104-weeks-old)
BioProject: PRJNA238328
Publication: PMID 24510058
Analysis date: Mon Jun 6 17:44:12 2016

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

High phosphofructokinase expression is associated with glioma.
Title: The up regulation of phosphofructokinase1 (PFK1) protein during chemically induced hypoxia is mediated by the hypoxia-responsive internal ribosome entry site (IRES) element, present in its 5'untranslated region.
Coordinated expression of PKF-A/FBPase controls sperm cell glucose metabolism and sperm development and function.
Title: Expression of key substrate cycle enzymes in rat spermatogenic cells: fructose 1,6 bisphosphatase and 6 phosphofructose 1-kinase.

Submit: New GeneRIF Correction

Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Markers

Pfkm (e-PCR), detects polymorphism
- UniSTS:261431

Pfkm (e-PCR), detects polymorphism
- UniSTS:507131

Gene Ontology Provided by RGD

Function	Evidence Code	Pubs
enables 6-phosphofructokinase activity	IBA Inferred from Biological aspect of Ancestor more info
enables 6-phosphofructokinase activity	IDA Inferred from Direct Assay more info	PubMed
enables 6-phosphofructokinase activity	IEA Inferred from Electronic Annotation more info
enables 6-phosphofructokinase activity	ISO Inferred from Sequence Orthology more info
enables 6-phosphofructokinase activity	ISS Inferred from Sequence or Structural Similarity more info
enables AMP binding	IBA Inferred from Biological aspect of Ancestor more info
enables AMP binding	IDA Inferred from Direct Assay more info	PubMed
enables ATP binding	IBA Inferred from Biological aspect of Ancestor more info
enables ATP binding	IDA Inferred from Direct Assay more info	PubMed
enables ATP binding	IEA Inferred from Electronic Annotation more info
enables ATP binding	ISO Inferred from Sequence Orthology more info
enables carbohydrate binding	IDA Inferred from Direct Assay more info	PubMed
enables fructose binding	IEA Inferred from Electronic Annotation more info
enables fructose binding	ISO Inferred from Sequence Orthology more info
enables fructose-6-phosphate binding	IBA Inferred from Biological aspect of Ancestor more info
enables fructose-6-phosphate binding	IDA Inferred from Direct Assay more info	PubMed
enables identical protein binding	IBA Inferred from Biological aspect of Ancestor more info
enables identical protein binding	IEA Inferred from Electronic Annotation more info
enables identical protein binding	IPI Inferred from Physical Interaction more info	PubMed
enables identical protein binding	ISO Inferred from Sequence Orthology more info
enables kinase binding	IEA Inferred from Electronic Annotation more info
enables kinase binding	ISO Inferred from Sequence Orthology more info
enables metal ion binding	IEA Inferred from Electronic Annotation more info
enables monosaccharide binding	IBA Inferred from Biological aspect of Ancestor more info
enables phosphofructokinase activity	IMP Inferred from Mutant Phenotype more info	PubMed
enables phosphofructokinase activity	ISO Inferred from Sequence Orthology more info
enables phosphofructokinase activity	NAS Non-traceable Author Statement more info	PubMed

Process	Evidence Code	Pubs
involved_in canonical glycolysis	IBA Inferred from Biological aspect of Ancestor more info
involved_in canonical glycolysis	IEA Inferred from Electronic Annotation more info
acts_upstream_of_or_within canonical glycolysis	ISO Inferred from Sequence Orthology more info
involved_in canonical glycolysis	ISO Inferred from Sequence Orthology more info
involved_in carbohydrate phosphorylation	IEA Inferred from Electronic Annotation more info
acts_upstream_of_or_within carbohydrate phosphorylation	ISO Inferred from Sequence Orthology more info
involved_in fructose 1,6-bisphosphate metabolic process	IBA Inferred from Biological aspect of Ancestor more info
involved_in fructose 1,6-bisphosphate metabolic process	IDA Inferred from Direct Assay more info	PubMed
involved_in fructose 6-phosphate metabolic process	IBA Inferred from Biological aspect of Ancestor more info
involved_in fructose 6-phosphate metabolic process	IDA Inferred from Direct Assay more info	PubMed
involved_in fructose 6-phosphate metabolic process	IEA Inferred from Electronic Annotation more info
involved_in fructose 6-phosphate metabolic process	ISO Inferred from Sequence Orthology more info
involved_in glucose homeostasis	IEA Inferred from Electronic Annotation more info
acts_upstream_of_or_within glucose homeostasis	ISO Inferred from Sequence Orthology more info
involved_in glycogen catabolic process	IEA Inferred from Electronic Annotation more info
acts_upstream_of_or_within glycogen catabolic process	ISO Inferred from Sequence Orthology more info
involved_in glycolysis from storage polysaccharide through glucose-1-phosphate	IEA Inferred from Electronic Annotation more info
acts_upstream_of_or_within glycolysis from storage polysaccharide through glucose-1-phosphate	ISO Inferred from Sequence Orthology more info
involved_in glycolytic process	IDA Inferred from Direct Assay more info	PubMed
involved_in glycolytic process	ISO Inferred from Sequence Orthology more info
involved_in glycolytic process through fructose-6-phosphate	IEA Inferred from Electronic Annotation more info
acts_upstream_of_or_within glycolytic process through fructose-6-phosphate	ISO Inferred from Sequence Orthology more info
involved_in glycolytic process through fructose-6-phosphate	ISO Inferred from Sequence Orthology more info
involved_in intracellular glucose homeostasis	NAS Non-traceable Author Statement more info	PubMed
involved_in muscle cell cellular homeostasis	IEA Inferred from Electronic Annotation more info
involved_in muscle cell cellular homeostasis	ISO Inferred from Sequence Orthology more info
involved_in positive regulation of insulin secretion	IEA Inferred from Electronic Annotation more info
acts_upstream_of_or_within positive regulation of insulin secretion	ISO Inferred from Sequence Orthology more info
involved_in positive regulation of transcription by RNA polymerase II	IEA Inferred from Electronic Annotation more info
involved_in positive regulation of transcription by RNA polymerase II	ISO Inferred from Sequence Orthology more info

Component	Evidence Code	Pubs
part_of 6-phosphofructokinase complex	IBA Inferred from Biological aspect of Ancestor more info
part_of 6-phosphofructokinase complex	IEA Inferred from Electronic Annotation more info
part_of 6-phosphofructokinase complex	ISO Inferred from Sequence Orthology more info
located_in apical plasma membrane	IEA Inferred from Electronic Annotation more info
located_in apical plasma membrane	ISO Inferred from Sequence Orthology more info
located_in cytoplasm	IEA Inferred from Electronic Annotation more info
is_active_in cytosol	ISO Inferred from Sequence Orthology more info
located_in cytosol	ISO Inferred from Sequence Orthology more info
is_active_in membrane	IBA Inferred from Biological aspect of Ancestor more info
located_in nucleus	IEA Inferred from Electronic Annotation more info
located_in nucleus	ISO Inferred from Sequence Orthology more info
located_in sperm principal piece	IEA Inferred from Electronic Annotation more info
located_in sperm principal piece	ISO Inferred from Sequence Orthology more info

General protein information

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Preferred Names: ATP-dependent 6-phosphofructokinase, muscle type

Names: 6-phosphofructokinase type A; 6-phosphofructokinase, muscle type; phosphofructo-1-kinase isozyme A; phosphofructokinase 1; phosphofructokinase-A; phosphofructokinase-M; phosphohexokinase

NP_113903.2

EC 2.7.1.11

XP_038935791.1

EC 2.7.1.11

XP_063120288.1

EC 2.7.1.11

XP_063120289.1

EC 2.7.1.11

XP_063120290.1

EC 2.7.1.11

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

NM_031715.2 → NP_113903.2 ATP-dependent 6-phosphofructokinase, muscle type

Status: VALIDATED

Source sequence(s)

JAXUCZ010000007

UniProtKB/Swiss-Prot

P47858, Q63736

UniProtKB/TrEMBL

A0A0G2JY38, A6KC58, Q52KS1

Related

ENSRNOP00000070507.1, ENSRNOT00000078579.2

Conserved Domains (1) summary

cd00764
Location:13 → 774

Eukaryotic_PFK; Phosphofructokinase, a key regulatory enzyme in glycolysis, catalyzes the phosphorylation of fructose-6-phosphate to fructose-1,6-biphosphate. The members belong to a subfamily of the PFKA family (cd00363) and include eukaryotic ATP-dependent ...

RefSeqs of Annotated Genomes: GCF_036323735.1-RS_2024_02

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference GRCr8

Genomic

NC_086025.1 Reference GRCr8

Range

131100684..131138250

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

XM_063264219.1 → XP_063120289.1 ATP-dependent 6-phosphofructokinase, muscle type isoform X1

UniProtKB/TrEMBL

A0A0G2KBC7
XM_039079863.2 → XP_038935791.1 ATP-dependent 6-phosphofructokinase, muscle type isoform X1

UniProtKB/TrEMBL

A0A0G2KBC7, A0A8I6ADR0

Related

ENSRNOP00000075742.1

Conserved Domains (1) summary

cd00764
Location:84 → 845

Eukaryotic_PFK; Phosphofructokinase, a key regulatory enzyme in glycolysis, catalyzes the phosphorylation of fructose-6-phosphate to fructose-1,6-biphosphate. The members belong to a subfamily of the PFKA family (cd00363) and include eukaryotic ATP-dependent ...
XM_063264220.1 → XP_063120290.1 ATP-dependent 6-phosphofructokinase, muscle type isoform X1

UniProtKB/TrEMBL

A0A0G2KBC7
XM_063264218.1 → XP_063120288.1 ATP-dependent 6-phosphofructokinase, muscle type isoform X1

UniProtKB/TrEMBL

A0A0G2KBC7