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    Fhl5 four and a half LIM domains 5 [ Mus musculus (house mouse) ]

    Gene ID: 57756, updated on 25-Apr-2024

    Summary

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    Official Symbol
    Fhl5provided by MGI
    Official Full Name
    four and a half LIM domains 5provided by MGI
    Primary source
    MGI:MGI:1913192
    See related
    Ensembl:ENSMUSG00000028259 AllianceGenome:MGI:1913192
    Gene type
    protein coding
    RefSeq status
    VALIDATED
    Organism
    Mus musculus
    Lineage
    Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus
    Also known as
    Act; 1700027G07Rik
    Summary
    Enables transcription coactivator activity. Acts upstream of or within positive regulation of transcription by RNA polymerase II. Predicted to be active in Z disc and nucleus. Is expressed in genitourinary system; heart; and otocyst. Orthologous to human FHL5 (four and a half LIM domains 5). [provided by Alliance of Genome Resources, Apr 2022]
    Expression
    Restricted expression toward testis adult (RPKM 61.6) See more
    Orthologs
    human all
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    Genomic context

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    Location:
    4 A3; 4 10.58 cM
    Exon count:
    8
    Annotation release Status Assembly Chr Location
    RS_2024_02 current GRCm39 (GCF_000001635.27) 4 NC_000070.7 (25199907..25242852, complement)
    108.20200622 previous assembly GRCm38.p6 (GCF_000001635.26) 4 NC_000070.6 (25199907..25242852, complement)

    Chromosome 4 - NC_000070.7Genomic Context describing neighboring genes Neighboring gene predicted gene, 46848 Neighboring gene NADH:ubiquinone oxidoreductase complex assembly factor 4 Neighboring gene RIKEN cDNA C230012O17 gene Neighboring gene G protein-coupled receptor 63 Neighboring gene UFM1 specific ligase 1 Neighboring gene peroxiredoxin 6 pseudogene 2

    Genomic regions, transcripts, and products

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    Go to nucleotide: Graphics FASTA GenBank

    Expression

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    • Project title: Mouse ENCODE transcriptome data
    • Description: RNA profiling data sets generated by the Mouse ENCODE project.
    • BioProject: PRJNA66167
    • Publication: PMID 25409824
    • Analysis date: n/a

    Bibliography

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    Related articles in PubMed

    1. Fhl5/Act, a CREM-binding transcriptional activator required for normal sperm maturation and morphology, is not essential for testicular gene expression. Lardenois A, et al. Reprod Biol Endocrinol, 2009 Nov 24. PMID 19930692, Free PMC Article
    2. Abnormal sperm in mice with targeted deletion of the act (activator of cAMP-responsive element modulator in testis) gene. Kotaja N, et al. Proc Natl Acad Sci U S A, 2004 Jul 20. PMID 15247423, Free PMC Article
    3. Cloning and expression of activator of CREM in testis in human testicular tissue. Palermo I, et al. Biochem Biophys Res Commun, 2001 May 4. PMID 11327716
    4. CREM-dependent transcription in male germ cells controlled by a kinesin. Macho B, et al. Science, 2002 Dec 20. PMID 12493914
    5. CBP-independent activation of CREM and CREB by the LIM-only protein ACT. Fimia GM, et al. Nature, 1999 Mar 11. PMID 10086359

    See all (24) citations in PubMed

    GeneRIFs: Gene References Into Functions

    What's a GeneRIF?
    1. Results indicate that SPAG8 acts as a regulator of ACT and plays an important role in CREM-ACT-mediated gene transcription during spermatogenesis.
      Title: Sperm associated antigen 8 (SPAG8), a novel regulator of activator of CREM in testis during spermatogenesis.
    2. Fhl5 is not essential for testicular gene expression to a level detected by microarray-based measurements.
      Title: Fhl5/Act, a CREM-binding transcriptional activator required for normal sperm maturation and morphology, is not essential for testicular gene expression.
    3. Results indicate that numerous postmeiotic genes under CREM (cAMP-responsive element modulator) control require the coactivator function of ACT (activator of CREM) in testis.
      Title: Abnormal sperm in mice with targeted deletion of the act (activator of cAMP-responsive element modulator in testis) gene.
    4. ACT selectively associates with KIF17b, a kinesin highly expressed in male germ cells; The ACT-KIF17b interaction is restricted to specific stages of spermatogenesis and directly determines the intracellular localization of ACT
      Title: CREM-dependent transcription in male germ cells controlled by a kinesin.
    Submit: New GeneRIF Correction

    Variation

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    Alleles

    Alleles of this type are documented at Mouse Genome Informatics  (MGI)
    • Endonuclease-mediated (2) 
    • Targeted (1)  1 citation

    Interactions

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    Products Interactant Other Gene Complex Source Pubs Description

    General gene information

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    Homology

    Gene Ontology Provided by MGI

    Function Evidence Code Pubs
    enables metal ion binding IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables protein binding IPI
    Inferred from Physical Interaction
    more info
    		 PubMed 
    enables transcription coactivator activity IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    enables transcription coactivator activity IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    Process Evidence Code Pubs
    acts_upstream_of_or_within DNA-templated transcription TAS
    Traceable Author Statement
    more info
    		 PubMed 
    involved_in positive regulation of transcription by RNA polymerase II IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    acts_upstream_of_or_within positive regulation of transcription by RNA polymerase II IGI
    Inferred from Genetic Interaction
    more info
    		 PubMed 
    Component Evidence Code Pubs
    is_active_in Z disc IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    is_active_in nucleus IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  

    General protein information

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    Preferred Names
    four and a half LIM domains protein 5
    Names
    FHL-5
    LIM-only protein ACT
    activator of CREM in testis
    activator of cAMP-responsive element modulator in testis

    NCBI Reference Sequences (RefSeq)

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    RefSeqs maintained independently of Annotated Genomes

    These reference sequences exist independently of genome builds. Explain

    These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

    mRNA and Protein(s)

    1. NM_001355498.2 → NP_001342427.1  four and a half LIM domains protein 5

      Status: VALIDATED

      Description
      Transcript Variant: This variant (2) encodes the same protein as variants 1 and 3.
      Source sequence(s)
      AL772199, AL935267
      Consensus CDS
      CCDS18009.1
      UniProtKB/Swiss-Prot
      Q9WTX7
      Conserved Domains (4) summary
      cd09343
      Location:37 → 95
      LIM1_FHL; The first LIM domain of Four and a half LIM domains protein (FHL)
      cd09347
      Location:222 → 277
      LIM4_FHL; The fourth LIM domain of Four and a half LIM domains protein (FHL)
      cd09428
      Location:102 → 155
      LIM2_FHL5; The second LIM domain of Four and a half LIM domains protein 5 (FHL5)
      cl02475
      Location:163 → 214
      LIM; LIM is a small protein-protein interaction domain, containing two zinc fingers

    2. NM_001379528.1 → NP_001366457.1  four and a half LIM domains protein 5

      Status: VALIDATED

      Description
      Transcript Variant: This variant (3) represents the longest transcript. All three variants encode the same protein.
      Source sequence(s)
      AL772199, AL935267
      UniProtKB/Swiss-Prot
      Q9WTX7
      Related
      ENSMUSP00000103839.2, ENSMUST00000108204.2
      Conserved Domains (4) summary
      cd09343
      Location:37 → 95
      LIM1_FHL; The first LIM domain of Four and a half LIM domains protein (FHL)
      cd09347
      Location:222 → 277
      LIM4_FHL; The fourth LIM domain of Four and a half LIM domains protein (FHL)
      cd09428
      Location:102 → 155
      LIM2_FHL5; The second LIM domain of Four and a half LIM domains protein 5 (FHL5)
      cl02475
      Location:163 → 214
      LIM; LIM is a small protein-protein interaction domain, containing two zinc fingers

    3. NM_021318.5 → NP_067293.1  four and a half LIM domains protein 5

      See identical proteins and their annotated locations for NP_067293.1

      Status: VALIDATED

      Description
      Transcript Variant: This variant (1) encodes the same protein as variants 2 and 3.
      Source sequence(s)
      AL772199, AL935267
      Consensus CDS
      CCDS18009.1
      UniProtKB/Swiss-Prot
      Q9WTX7
      Related
      ENSMUSP00000029922.8, ENSMUST00000029922.14
      Conserved Domains (4) summary
      cd09343
      Location:37 → 95
      LIM1_FHL; The first LIM domain of Four and a half LIM domains protein (FHL)
      cd09347
      Location:222 → 277
      LIM4_FHL; The fourth LIM domain of Four and a half LIM domains protein (FHL)
      cd09428
      Location:102 → 155
      LIM2_FHL5; The second LIM domain of Four and a half LIM domains protein 5 (FHL5)
      cl02475
      Location:163 → 214
      LIM; LIM is a small protein-protein interaction domain, containing two zinc fingers

    RefSeqs of Annotated Genomes: GCF_000001635.27-RS_2024_02

    The following sections contain reference sequences that belong to a specific genome build. Explain

    Reference GRCm39 C57BL/6J

    Genomic

    1. NC_000070.7 Reference GRCm39 C57BL/6J

      Range
      25199907..25242852 complement
      Download
      GenBank, FASTA, Sequence Viewer (Graphics)
    Nucleotide Protein Strain
    Heading Accession and Version
    Protein Accession Links
    GenPept Link UniProtKB Link
    Q9WTX7.1 GenPept UniProtKB/Swiss-Prot:Q9WTX7

    Gene LinkOut

    The following LinkOut resources are supplied by external providers. These providers are responsible for maintaining the links.

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