GTR Home > Tests > HER2 FISH analysis


Test order codeHelp: 003019

Test name


HER2 FISH analysis

Purpose of the test


This is a clinical test intended for Help: Diagnosis, Drug Response, Monitoring, Prognostic, Therapeutic management



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How to order


Complete requisition form
Order URL Help:

Specimen source

Peripheral (whole) blood
Specimen requirements:


Fluorescent in situ hybridization
  • None/not applicable

Summary of what is tested

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Clinical utility


Guidance for management

  • Safety and efficacy of everolimus with exemestane vs. exemestane alone in elderly patients with HER2-negative, hormone receptor-positive breast cancer in BOLERO-2. - PubMed ID: 24267730
  • Eur J Cancer. 2013 Nov 21. doi:pii: S0959-8049(13)00904-0. 10.1016/j.ejca.2013.10.004

Clinical validity


Control probes are used in each case.


Not provided

Testing strategy


Fluorescent in situ hybridization of HER. Normal controls used at the same time. 000 Complete requisition form

Test services

  • Clinical Testing/Confirmation of Mutations Identified Previously
  • Genetic counseling

Suggested reading

  • Cardoso et al., 2016
  • Ward et al., 2013
    Gene expression profiling and expanded immunohistochemistry tests to guide the use of adjuvant chemotherapy in breast cancer management: a systematic review and cost-effectiveness analysis.

Clinical resources

Practice guidelines

  • NICE, 2024
    UK NICE Guideline NG101, Early and locally advanced breast cancer: diagnosis and management, 2024
  • NCCN, 2023
    Breast Cancer, NCCN Guidelines Version 4.2022
  • NCCN, 2022
    Breast Cancer Screening and Diagnosis, NCCN, Version 1.2022
  • NICE, 2018
    Tumour profiling tests to guide adjuvant chemotherapy decisions in early breast cancer (DG34)
  • NICE, 2018
    Tumour profiling tests to guide adjuvant chemotherapy decisions in early breast cancer, NICE, 2018
  • NCCN, 2014
    National Comprehensive Cancer Network Clinical Practice Guidelines in Oncology. Breast Cancer (See 2022 Update)
  • NICE, 2013
    DG10 Gene expression profiling and expanded immunohistochemistry tests for guiding adjuvant chemotherapy decisions in early breast cancer management: MammaPrint, Oncotype DX, IHC4 and Mammostrat: information for the public
  • AHRQ, 2013
    Technology Assessment on Genetic Testing or Molecular Pathology Testing of Cancers with Unknown Primary Site to Determine Origin (ARCHIVED)
  • NCCN, 2011
    NCCN Task Force report: Evaluating the clinical utility of tumor markers in oncology.

Consumer resources

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