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MGMT Promoter Methylation assay
Clinical Genetic Test
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offered by
UCSF Molecular Diagnostics Laboratory
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GTR Test Accession: Help GTR000529070.6
CANCERNERVOUS SYSTEM
Last updated in GTR: 2021-10-26
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Last annual review date for the lab: 2022-10-27 Past due LinkOut
At a Glance
Test purpose: Help
Prognostic
Conditions (1): Help
Glioblastoma
Genes (1): Help
MGMT (10q26.3)
Methods (1): Help
Molecular Genetics - Methylation analysis: Next Generation Sequencing
Target population: Help
Patients with glioblastoma
Clinical validity: Help
Not provided
Clinical utility: Help
Guidance for selecting a drug therapy and/or dose
Ordering Information
Offered by: Help
UCSF Molecular Diagnostics Laboratory
View lab's website
View lab's test page
Test short name: Help
MGMT
Specimen Source: Help
Who can order: Help
  • Licensed Physician
Test Order Code: Help
MGMT
Lab contact: Help
Farid Chehab, PhD, MB(ASCP), Lab Director
farid.chehab@ucsf.edu
415-476-0310
Contact Policy: Help
Laboratory can only accept contact from health care providers. Patients/families are encouraged to discuss genetic testing options with their health care provider.
How to Order: Help
Order URL
Test development: Help
Test developed by laboratory (no manufacturer test name)
Informed consent required: Help
Decline to answer
Pre-test genetic counseling required: Help
Decline to answer
Post-test genetic counseling required: Help
Decline to answer
Recommended fields not provided:
Test strategy
Conditions Help
Total conditions: 1
Condition/Phenotype Identifier
Test Targets
Genes Help
Total genes: 1
Gene Associated Condition Germline or Somatic Allele (Lab-provided) Variant in NCBI
Methodology
Total methods: 1
Method Category Help
Test method Help
Instrument *
Methylation analysis
Next Generation Sequencing
* Instrument: Not provided
Clinical Information
Test purpose: Help
Prognostic
Clinical utility: Help
Guidance for selecting a drug therapy and/or dose
View citations (1)
  • MGMT gene silencing and benefit from temozolomide in glioblastoma. Hegi ME, et al. N Engl J Med. 2005;352(10):997-1003. doi:10.1056/NEJMoa043331. PMID: 15758010.

Target population: Help
Patients with glioblastoma
Recommended fields not provided:
Clinical validity, What is the protocol for interpreting a variation as a VUS?, Are family members with defined clinical status recruited to assess significance of VUS without charge?, Will the lab re-contact the ordering physician if variant interpretation changes?, Is research allowed on the sample after clinical testing is complete?, Sample negative report, Sample positive report
Technical Information
Test Comments: Help
The addition of a methyl group to the O6 position of guanine is a mutagenic event that is corrected by a DNA repair protein encoded by the MGMT gene. If the MGMT repair mechanism fails to occur, cells will undergo apoptosis and thus become more susceptible to drug induced-cytotoxicity. In … View more
Availability: Help
Tests performed
Entire test performed in-house
Analytical Validity: Help
Methylation of 17 individual CpG methylation sites across a promoter region of 191 bp is determined by Next Generation Sequencing.
Assay limitations: Help
Infiltrating normal tissue in tumor sections may reduce the detection of methylation CpG sites. Tumor-rich areas should be circled on an H&E stained paraffin section.
Proficiency testing (PT):
Is proficiency testing performed for this test? Help
Yes

Method used for proficiency testing: Help
Intra-Laboratory
Recommended fields not provided:
Test Confirmation, Citations to support assay limitations, Description of internal test validation method, Citations for Analytical validity, PT Provider, Description of PT method, Major CAP category, CAP category, CAP test list
Regulatory Approval
FDA Review: Help
Category: FDA exercises enforcement discretion
Additional Information

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