Alternative titles; symbols
HGNC Approved Gene Symbol: MAGEA12
Cytogenetic location: Xq28 Genomic coordinates (GRCh38): X:152,733,757-152,737,669 (from NCBI)
Genes of the MAGE family direct the expression of tumor antigens that are recognized on a human melanoma by autologous cytolytic T lymphocytes. Family A (see 300016) is clustered at Xq28 and family B (see 300097) is clustered at Xp21.3.
De Plaen et al. (1994) identified 12 family A MAGE genes. MAGE12 cDNA was cloned from a melanoma cell cDNA library. The gene encodes a 314-amino acid polypeptide. RT-PCR revealed that MAGE12 is expressed in a variety of cancer cell lines, but among normal tissues only in testis.
De Plaen et al. (1994) determined that the MAGEA12 gene comprises 3 exons, with the last exon containing the entire coding sequence.
De Plaen et al. (1994) used human/rodent cell hybrids to map the MAGE family A cluster to Xq26-qter. Rogner et al. (1995) refined the mapping of the MAGE family A cluster to Xq28. The 12 genes are arranged in 3 clusters within 3.5 Mb.
See 300016 for a discussion of the high frequency of genes on the X chromosome encoding proteins with the MAGE domain as well as other cancer-testis antigen genes (Ross et al., 2005).
De Plaen, E., Arden, K., Traversari, C., Gaforio, J. J., Szikora, J.-P., De Smet, C., Brasseur, F., van der Bruggen, P., Lethe, B., Lurquin, C., Brasseur, R., Chomez, P., De Backer, O., Cavenee, W., Boon, T. Structure, chromosomal localization, and expression of 12 genes of the MAGE family. Immunogenetics 40: 360-369, 1994. [PubMed: 7927540] [Full Text: https://doi.org/10.1007/BF01246677]
Rogner, U. C., Wilke, K., Steck, E., Korn, B., Poustka, A. The melanoma antigen gene (MAGE) family is clustered in the chromosomal band Xq28. Genomics 29: 725-731, 1995. [PubMed: 8575766] [Full Text: https://doi.org/10.1006/geno.1995.9945]
Ross, M. T., Grafham, D. V., Coffey, A. J., Scherer, S., McLay, K., Muzny, D., Platzer, M., Howell, G. R., Burrows, C., Bird, C. P., Frankish, A., Lovell, F. L., and 270 others. The DNA sequence of the human X chromosome. Nature 434: 325-337, 2005. [PubMed: 15772651] [Full Text: https://doi.org/10.1038/nature03440]