Alternative titles; symbols
HGNC Approved Gene Symbol: CYP2J2
Cytogenetic location: 1p32.1 Genomic coordinates (GRCh38): 1:59,893,308-59,969,212 (from NCBI)
Wu et al. (1996) isolated a cDNA from a human liver library using a rabbit clone for the P450 gene CYP2J1 (Kikuta et al., 1991) as a probe. The 1.9-kb cDNA encoded a predicted 502-amino acid protein contained a putative heme binding domain and was 80% identical to the rabbit CYP2J1 protein. Because of these similarities the authors designated the human protein CYP2J2. The protein was most similar (40-46%) to hemoproteins of the CYP2 family and, to a lesser extent, other CYP families. Northern blots showed the strongest expression of CYP2J2 mRNA to be in the heart, with lower levels in the liver, ileum, jejunum, colon, and kidney; antibodies raised against the protein were used to confirm that the predominant expression was in the heart. Wu et al. (1996) also found that recombinantly expressed protein had enzymatic activity.
Watanabe et al. (2003) demonstrated that the endocannabinoid anandamide and its metabolite arachidonic acid activate TRPV4 (605427) in an indirect way involving the cytochrome p450 epoxygenase-dependent formation of epoxyeicosatrienoic acids. Application of 5-prime,6-prime-epoxyeicosatrienoic acid at submicromolar concentrations activated TRPV4 in a membrane-delimited manner and caused calcium influx through TRPV4-like channels in vascular endothelial cells. Watanabe et al. (2003) concluded that activation of TRPV4 in vascular endothelial cells might contribute to the relaxant effects of endocannabinoids and their p450 epoxygenase-dependent metabolites on vascular tone.
Ma et al. (1998) mapped the CYP2J2 gene to human chromosome 1 on a panel of somatic hybrid cell lines and to 1p31.3-p31.2 by fluorescence in situ hybridization.
Kikuta, Y., Sogawa, K., Haniu, M., Kinosaki, M., Kusunose, E., Nojima, Y., Yamamoto, S., Ichihara, K., Kusunose, M., Fujii-Kuriyama, Y. A novel species of cytochrome P-450 (P-450ib) specific for the small intestine of rabbits: cDNA cloning and its expression in COS cells. J. Biol. Chem. 266: 17821-17825, 1991. [PubMed: 1717443]
Ma, J., Ramachandran, S., Fiedorek, F. T., Jr., Zeldin, D. C. Mapping of the CYP2J cytochrome P450 genes to human chromosome 1 and mouse chromosome 4. Genomics 49: 152-155, 1998. [PubMed: 9570962] [Full Text: https://doi.org/10.1006/geno.1998.5235]
Watanabe, H., Vriens, J., Prenen, J., Droogmans, G., Voets, T., Nilius, B. Anandamide and arachidonic acid use epoxyeicosatrienoic acids to activate TRPV4 channels. Nature 424: 434-438, 2003. [PubMed: 12879072] [Full Text: https://doi.org/10.1038/nature01807]
Wu, S., Moomaw, C. R., Tomer, K. B., Falck, J. R., Zeldin, D. C. Molecular cloning and expression of CYP2J2, a human cytochrome P450 arachidonic acid epoxygenase highly expressed in heart. J. Biol. Chem. 271: 3460-3468, 1996. [PubMed: 8631948] [Full Text: https://doi.org/10.1074/jbc.271.7.3460]