Alternative titles; symbols
HGNC Approved Gene Symbol: RIDA
Cytogenetic location: 8q22.2 Genomic coordinates (GRCh38): 8:98,102,344-98,117,171 (from NCBI)
Oka et al. (1995) identified a 14.5-kD protein that was coextracted with H1 (see 142709) and HMG (see 163906) proteins from rat liver by 5% perchloric acid, and they designated it PSP for 'perchloric acid-soluble protein.' They cloned the rat gene by screening a liver cDNA expression library with anti-PSP antibodies. The PSP gene encodes a predicted 136-amino acid protein that inhibits protein synthesis at the initiation stage in a cell-free system. Using Northern and Western blots, Oka et al. (1995) found that PSP is expressed only in liver and kidney. Native PSP is a homodimer. Ceciliani et al. (1996) identified the goat homolog of PSP as UK114, 1 of 3 major protein components of UK101, a tumor antigen that is expressed by many human malignant neoplasms.
Schmiedeknecht et al. (1996) found the human homolog of PSP by studying proteins that are upregulated during monocyte/macrophage differentiation. They used RT-PCR with primers based on rat PSP to clone the human gene from mononuclear phagocyte mRNA, and designated it p14.5. The predicted 137-amino acid protein is 82% identical to rat PSP and is similar to a hypothetical family (YER057c/YJGF) of small (15-kD) proteins found in both prokaryotes and eukaryotes, all of which have a highly conserved C-terminal domain. p14.5 inhibits in vitro protein synthesis. On Northern blots, p14.5 is expressed as an approximately 1-kb mRNA, and is found primarily in liver and kidney. Using immunohistochemistry, Schmiedeknecht et al. (1996) found that p14.5 was present mostly in the cytoplasm in liver, kidney and vessel wall sections, with only hepatocytes, distal tubular cells, endothelial cells and smooth muscle cells showing strong staining.
Schmiedeknecht et al. (1997) mapped the p14.5 gene to 8q22 by fluorescence in situ hybridization. They found that p14.5 and POP1 (602486) are oriented head-to-head, separated by a 102-bp region that contains a bidirectional promoter.
Ceciliani, F., Faotto, L., Negri, A., Colombo, I., Berra, B., Bartorelli, A., Ronchi, S. The primary structure of UK114 tumor antigen. FEBS Lett. 393: 147-150, 1996. [PubMed: 8814279] [Full Text: https://doi.org/10.1016/0014-5793(96)00850-2]
Oka, T., Tsuji, H., Noda, C., Sakai, K., Hong, Y., Suzuki, I., Munoz, S., Natori, Y. Isolation and characterization of a novel perchloric acid-soluble protein inhibiting cell-free protein synthesis. J. Biol. Chem. 270: 30060-30067, 1995. [PubMed: 8530410] [Full Text: https://doi.org/10.1074/jbc.270.50.30060]
Schmiedeknecht, G., Buchler, C., Schmitz, G. A bidirectional promoter connects the p14.5 gene to the gene for RNase P and RNase MRP protein subunit hPOP1. Biochem. Biophys. Res. Commun. 241: 59-67, 1997. [PubMed: 9405234] [Full Text: https://doi.org/10.1006/bbrc.1997.7772]
Schmiedeknecht, G., Kerkhoff, C., Orso, E., Stohr, J., Aslanidis, C., Nagy, G. M., Knuechel, R., Schmitz, G. Isolation and characterization of a 14.5-kDa trichloroacetic-acid-soluble translational inhibitor protein from human monocytes that is upregulated upon cellular differentiation. Europ. J. Biochem. 242: 339-351, 1996. [PubMed: 8973653] [Full Text: https://doi.org/10.1111/j.1432-1033.1996.0339r.x]