HGNC Approved Gene Symbol: ZNF174
Cytogenetic location: 16p13.3 Genomic coordinates (GRCh38): 16:3,401,215-3,409,364 (from NCBI)
Members of the Cys2-His2 zinc finger class of transcription factors, such as ZNF174, appear to be involved in the transcriptional repression of growth factor gene expression (summary by Williams et al., 1995).
To identify novel genes that can repress the expression of growth regulatory molecules, Williams et al. (1995) screened a human fetal cDNA library with a degenerate oligonucleotide corresponding to the conserved 'knuckle' region, or H/C link, that connects successive zinc fingers of the WT1 (607102)/EGR1 (128990) family of DNA-binding proteins. They isolated overlapping partial cDNAs which generated an open reading frame encoding a 407-amino acid protein with a calculated molecular mass of 46,414 Da. The predicted ZNF174 protein contains 3 C-terminal tandem zinc fingers, a single potential phosphorylation site, a putative nuclear localization signal, and an N-terminal element termed the SCAN box, which is also found in a number of other zinc finger proteins. Northern blot analysis detected a major 2.5-kb ZNF174 transcript in a wide variety of adult human tissues, with the highest levels found in ovary and testis.
Williams et al. (1995) demonstrated that recombinant ZNF174 can repress expression driven by the promoters of the PDGFB (190040) and TGFB1 (190180) genes.
Williams et al. (1995) localized the ZNF174 gene to 16p13.3 by somatic cell hybrid mapping and fluorescence in situ hybridization.
Williams, A. J., Khachigian, L. M., Shows, T., Collins, T. Isolation and characterization of a novel zinc-finger protein with transcriptional repressor activity. J. Biol. Chem. 270: 22143-22152, 1995. [PubMed: 7673192] [Full Text: https://doi.org/10.1074/jbc.270.38.22143]