Alternative titles; symbols
HGNC Approved Gene Symbol: AKAP11
Cytogenetic location: 13q14.11 Genomic coordinates (GRCh38): 13:42,271,477-42,323,261 (from NCBI)
A-kinase anchor proteins (AKAPs; see 602449) direct the activity of protein kinase A (PKA; see 176911) by tethering the enzyme near its physiologic substrates.
By screening rat pituitary and olfactory bulb cDNA libraries with a PKA type II regulatory (RII) probe, Lester et al. (1996) identified a novel AKAP, AKAP11, which the authors designated AKAP220. The AKAP11 protein has 1,129 amino acids and contains a PKA-binding region and a peroxisome targeting motif. Immunohistochemical analysis of rat testis cells showed that AKAP220 and a proportion of RII PKA are colocalized in microbodies that appear to be a subset of peroxisomes. By RT-PCR analysis, Ishikawa et al. (1998) detected expression of human AKAP11 in heart, brain, lung, liver, kidney, testis, and ovary, with weaker expression in skeletal muscle, pancreas, and spleen.
Logue et al. (2011) showed that AKAP220 interacts with the cytoskeletal scaffolding protein IQGAP1 (603379) at the leading edges of migrating cells. The AKAP220/IQGAP1 complex was proposed to form a component of a microtubule-associated network of several proteins, including CLASP2 (605853), that receive and process calcium and cAMP second messenger signals. Silencing of the AKAP220 gene retarded cell migration in human cancer cells.
By radiation hybrid analysis, Ishikawa et al. (1998) mapped the AKAP11 gene, which they designated KIAA0629, to human chromosome 13.
Ishikawa, K., Nagase, T., Suyama, M., Miyajima, N., Tanaka, A., Kotani, H., Nomura, N., Ohara, O. Prediction of the coding sequences of unidentified human genes. X. The complete sequences of 100 new cDNA clones from brain which can code for large proteins in vitro. DNA Res. 5: 169-176, 1998. [PubMed: 9734811] [Full Text: https://doi.org/10.1093/dnares/5.3.169]
Lester, L. B., Coghlan, V. M., Nauert, B., Scott, J. D. Cloning and characterization of a novel A-kinase anchoring protein: AKAP 220, association with testicular peroxisomes. J. Biol. Chem. 271: 9460-9465, 1996. [PubMed: 8621616] [Full Text: https://doi.org/10.1074/jbc.271.16.9460]
Logue, J. S., Whiting, J. L., Tunquist, B., Sacks, D. B., Langeberg, L. K., Wordeman, L., Scott, J. D. AKAP220 protein organizes signaling elements that impact cell migration. J. Biol. Chem. 286: 39269-39281, 2011. [PubMed: 21890631] [Full Text: https://doi.org/10.1074/jbc.M111.277756]