Alternative titles; symbols
HGNC Approved Gene Symbol: MRPL28
Cytogenetic location: 16p13.3 Genomic coordinates (GRCh38): 16:366,969-370,538 (from NCBI)
Mitochondria have their own translation system for production of 13 inner membrane proteins essential for oxidative phosphorylation. MRPL28 is a component of the large subunit of the mitochondrial ribosome that is encoded by the nuclear genome (Koc et al., 2001).
Treatment with tumor-infiltrating lymphocytes (TILs) and interleukin-2 (IL2; 147680) can mediate regression of metastatic melanoma (Rosenberg et al., 1990). TILs recognize and lyse melanoma cells in an MHC class I- or class II-restricted manner. The antigens may be encoded by nonmutated genes in which the level of expression is increased in melanoma. Robbins et al. (1995) obtained a TIL clone, TIL 1290, from a patient with a recurrent pelvic tumor 3 years after successful treatment with another TIL clone, TIL 888, which recognizes tyrosinase in an HLA-A24-restricted manner. They also obtained a melanoma cell line, 888 mel, from the same patient. By screening an 888 mel cDNA library with TIL 1290 for induction of cytokine GMCSF (138960) release, Robbins et al. (1995) isolated a cDNA that specifically stimulated TIL 1290 in an HLA-A24-restricted manner, but not TIL 888. The cDNA encodes a deduced 128-amino acid protein, MAAT1, termed p15 by the authors. The lack of a signal peptide, N-linked glycosylation sites, and hydrophobic domains suggests that MAAT1 is a cytoplasmic or nuclear protein. Northern blot analysis revealed expression of an approximately 0.9-kb transcript in all normal tissues and cell lines examined.
By searching databases using bovine Mrpl28 as query, Koc et al. (2001) identified human MRPL28. The deduced 256-amino acid protein has an N-terminal mitochondrial import signal with a cleavage site at residue 25. Koc et al. (2001) also identified MRPL28 homologs in all species examined, including plants. Mouse and human MRPL28 share 82.8% amino acid identity.
The International Radiation Hybrid Mapping Consortium mapped the MRPL28 gene to chromosome 16 (REN95718).
Koc, E. C., Burkhart, W., Blackburn, K., Moyer, M. B., Schlatzer, D. M., Moseley, A., Spremulli, L. L. The large subunit of the mammalian mitochondrial ribosome: analysis of the complement of ribosomal proteins present. J. Biol. Chem. 276: 43958-43969, 2001. [PubMed: 11551941] [Full Text: https://doi.org/10.1074/jbc.M106510200]
Robbins, P. F., El-Gamil, M., Li, Y. F., Topalian, S. L., Rivoltini, L., Sakaguchi, K., Appella, E., Kawakami, Y., Rosenberg, S. A. Cloning of a new gene encoding an antigen recognized by melanoma-specific HLA-A24-restricted tumor-infiltrating lymphocytes. J. Immun. 154: 5944-5950, 1995. [PubMed: 7751637]
Rosenberg, S. A., Aebersold, P., Cornetta, K., Kasid, A., Morgan, R. A., Moen, R., Karson, E. M., Lotze, M. T., Yang, J. C., Topalian, S. L., Merino, M. J., Culver, K., Miller, A. D., Blaese, R. M., Anderson, W. F. Gene transfer into humans: immunotherapy of patients with advanced melanoma, using tumor-infiltrating lymphocytes modified by retroviral gene transduction. New Eng. J. Med. 323: 570-578, 1990. [PubMed: 2381442] [Full Text: https://doi.org/10.1056/NEJM199008303230904]