Alternative titles; symbols
HGNC Approved Gene Symbol: TRIM34
Cytogenetic location: 11p15.4 Genomic coordinates (GRCh38): 11:5,619,944-5,644,398 (from NCBI)
RING finger proteins play crucial roles in differentiation, development, oncogenesis, and apoptosis. Although RING finger domains are involved in protein-protein interactions and typically bind zinc, they are distinct from zinc finger domains in terms of sequence and structure. Using PCR with degenerate primers to generate probes for novel RING finger proteins, followed by screening placenta and ovary cDNA libraries, Orimo et al. (2000) isolated cDNAs encoding 3 isoforms of RNF21. The deduced 842-amino acid long form contains 2 RING finger domains, 2 RING finger B-box coiled-coil (RBCC) domains, and a C-terminal B30.2 domain. The 488-amino acid medium form has 1 of each of these domains, whereas the 270-amino acid short form has a RING finger domain and a RBCC domain but lacks the B30.2 domain. Immunoblot analysis detected 98-, 54-, and 31-kD proteins. RT-PCR analysis indicated that each isoform is expressed in placenta and testis. Northern blot analysis revealed expression of a 4-kb transcript predominantly in testis and a 2.4-kb transcript that was highly expressed in placenta, spleen, colon, and blood leukocytes. The long form was expressed only in testis, kidney, and ovary. Expression of the medium form was upregulated in response to alpha-interferon (see 147660) and, later, to gamma-interferon (147570).
By FISH, Orimo et al. (2000) mapped the RNF21 gene to 11p15.4-p15.3.
Orimo, A., Tominaga, N., Yoshimura, K., Yamauchi, Y., Nomura, M., Sato, M., Nogi, Y., Suzuki, M., Suzuki, H., Ikeda, K., Inoue, S., Muramatsu, M. Molecular cloning of ring finger protein 21 (RNF21)/interferon-responsive finger protein (ifp1), which possesses two RING-B box-coiled coil domains in tandem. Genomics 69: 143-149, 2000. [PubMed: 11013086] [Full Text: https://doi.org/10.1006/geno.2000.6318]