Alternative titles; symbols
HGNC Approved Gene Symbol: CLEC10A
Cytogenetic location: 17p13.1 Genomic coordinates (GRCh38): 17:7,074,537-7,080,251 (from NCBI)
Members of the calcium-dependent (C-type) lectin family, such as CLEC10A, recognize carbohydrate chains and are involved in host defense mechanisms (Suzuki et al., 1996).
By screening an interleukin-2 (IL2; 147680)-activated peripheral blood monocyte cDNA library with a hepatic asialoglycoprotein receptor (see 108360) probe, Suzuki et al. (1996) isolated a cDNA encoding HML (human macrophage lectin). The predicted 292-amino acid type II transmembrane protein has a cytoplasmic domain with a tyr-glu-asn-phe (YENF) internalization signal, a transmembrane domain, a neck domain, and a C-type lectin domain (also called a carbohydrate recognition domain, or CRD) containing a gln-pro-asp motif. Western blot analysis showed that HML had an apparent molecular mass of 35 kD.
Functional analysis by Suzuki et al. (1996) indicated that HML bound specifically to galactose or N-acetylgalactosamine in the presence of calcium.
Gross (2010) mapped the CLEC10A gene to chromosome 17p13.1 based on an alignment of the CLEC10A sequence (GenBank BC027858) with the genomic sequence (GRCh37).
Mgl1 and Mgl2 are mouse homologs of human MGL that have distinct binding specificities. Westcott et al. (2009) examined the impact of diet-induced obesity on inflammation and metabolism in mice lacking Mgl1, a marker of alternatively activated macrophages. Mgl1 was not required for trafficking of antiinflammatory type-2 adipose tissue macrophages to adipose tissue. Obese Mgl1 -/- mice were protected from glucose intolerance, insulin resistance, and steatosis, despite having more visceral fat, and this protection was associated with significantly reduced inflammatory type-1 Cd11c (ITGAX; 151510)-positive macrophages. FACS analysis revealed that Mgl1 was expressed on inflammatory 7/4(hi) monocytes, and obese Mgl1 -/- mice had lower levels of 7/4(hi) monocytes. Westcott et al. (2009) concluded that Mgl1 is a cell surface receptor involved in the regulation of monocyte/macrophage activation and trafficking to fat in obesity.
Gross, M. B. Personal Communication. Baltimore, Md. 7/7/2010.
Suzuki, N., Yamamoto, K., Toyoshima, S., Osawa, T., Irimura, T. Molecular cloning and expression of cDNA encoding human macrophage C-type lectin: its unique carbohydrate binding specificity for Tn antigen. J. Immun. 156: 128-135, 1996. [PubMed: 8598452]
Westcott, D. J., DelProposto, J. B., Geletka, L. M., Wang, T., Singer, K., Saltiel, A. R., Lumeng, C. N. MGL1 promotes adipose tissue inflammation and insulin resistance by regulating 7/4(hi) monocytes in obesity. J. Exp. Med. 206: 3143-3156, 2009. [PubMed: 19995956] [Full Text: https://doi.org/10.1084/jem.20091333]