Alternative titles; symbols
HGNC Approved Gene Symbol: POP4
Cytogenetic location: 19q12 Genomic coordinates (GRCh38): 19:29,606,283-29,617,237 (from NCBI)
Ribonuclease P (RNase P) removes the 5-prime leader sequences from precursor tRNA molecules. RNase P consists of an RNA species (H1 RNA), the POP1 protein (602486), and at least 7 proteins called RPPs. The RPPs have apparent molecular masses of 14 kD (RPP14; 606112), 20 kD (RPP20; 606113), 25 kD (RPP25; 619235), 29 kD (RPP29), 30 kD (RPP30; 606115), 38 kD (RPP38; 606116), and 40 kD (RPP40; 606117). Patients with scleroderma (181750) have serum reactive with RNase P, the Th antigen, which is also referred to as the To antigen, and RPP30 and RPP38 (summary by Jarrous et al., 1998 and Jarrous et al., 1999).
By biochemical purification of RNase P, micropeptide sequence analysis, and EST database searching, Jarrous et al. (1999) obtained a cDNA encoding RPP29. The deduced 220-amino acid basic protein is rich in arginine and lysine, has nuclear localization sequences, and has a predicted molecular mass of approximately 25 kD. RPP29 is 23% identical to the yeast Pop4 protein. Fluorescent microscopy demonstrated nuclear and nucleolar expression of RPP29. Immunoblot analysis showed that antibodies to RPP14 or RPP29 precipitate native RNase P.
Van Eenennaam et al. (1999) cloned human and mouse RPP29, which they termed POP4. In addition to RNase P, immunoprecipitation analysis indicated that RPP29 is associated with RNase MRP (see 157660).
Gross (2013) mapped the POP4 gene to chromosome 19q12 based on an alignment of the POP4 sequence (GenBank AF001176) with the genomic sequence (GRCh37).
Gross, M. B. Personal Communication. Baltimore, Md. 9/9/2013.
Jarrous, N., Eder, P. S., Guerrier-Takada, C., Hoog, C., Altman, S. Autoantigenic properties of some protein subunits of catalytically active complexes of human ribonuclease P. RNA 4: 407-417, 1998. [PubMed: 9630247]
Jarrous, N., Eder, P. S., Wesolowski, D., Altman, S. Rpp14 and Rpp29, two protein subunits of human ribonuclease P. RNA 5: 153-157, 1999. [PubMed: 10024167] [Full Text: https://doi.org/10.1017/s135583829800185x]
van Eenennaam, H., Pruijn, G. J. M., van Venrooij, W. J. hPop4: a new protein subunit of the human RNase MRP and RNase P ribonucleoprotein complexes. Nucleic Acids Res. 27: 2465-2472, 1999. [PubMed: 10352175] [Full Text: https://doi.org/10.1093/nar/27.12.2465]