* 606356

TRANSMEMBRANE PROTEIN 123; TMEM123


Alternative titles; symbols

PRO-ONCOSIS RECEPTOR INDUCING MEMBRANE INJURY; PORIMIN
KERATINOCYTE-ASSOCIATED TRANSMEMBRANE PROTEIN 3; KCT3


HGNC Approved Gene Symbol: TMEM123

Cytogenetic location: 11q22.2     Genomic coordinates (GRCh38): 11:102,396,332-102,452,765 (from NCBI)


TEXT

Description

PORIMIN (pro-oncosis receptor inducing membrane injury), a member of the cell membrane-associated mucin family, mediates oncosis-like cell death.


Cloning and Expression

Zhang et al. (1998) reported the generation and characterization of a monoclonal antibody, designated anti-PORIMIN (pro-oncosis receptor inducing membrane injury), which mediated oncosis-like cell death in Jurkat cells. Anti-PORIMIN-triggered cell death showed no evidence of DNA fragmentation or formation of apoptotic bodies but rapidly showed plasma membrane injury followed by cell death. Ma et al. (2001) isolated PORIMIN cDNA from a Jurkat cell cDNA library by COS cell expression cloning. The cDNA encodes a deduced 189-amino acid type I transmembrane protein. Its extracellular domain contains many O-linked and 7 N-linked glycosylation sites that define it as a member of the mucin family. Northern blot analysis detected ubiquitous expression of a major 3.3-kb transcript, with comparable and relatively high levels in trachea, fetal kidney, uterus, thyroid gland, adult liver, and 2 tumor cell lines, colorectal adenocarcinoma SW480 and lung carcinoma A549. The only tissue that did not express PORIMIN was ovary. COS-7 and 293 cells transiently transfected with PORIMIN cDNA were specifically recognized by anti-PORIMIN antibody in cell staining and immunoblotting experiments.


Gene Structure

Ma et al. (2001) determined that the PORIMIN gene contains 4 exons and spans approximately 133 kb of genomic DNA.


Mapping

By sequence analysis, Ma et al. (2001) mapped the TMEM123 gene to chromosome 11q22.1.


History

The term oncosis, derived from the Greek 'onkos' (meaning swelling), was first used in 1910 to describe ischemic cell death in osteocytes and was later used by Trump et al. (1997) to describe a form of cell death distinct from apoptosis.


REFERENCES

  1. Ma, F., Zhang, C., Prasad, K. V. S., Freeman, G. J., Schlossman, S. F. Molecular cloning of porimin, a novel cell surface receptor mediating oncotic cell death. Proc. Nat. Acad. Sci. 98: 9778-9783, 2001. [PubMed: 11481458, images, related citations] [Full Text]

  2. Trump, B. F., Berezesky, I. K., Chang, S. H., Phelps, P. C. The pathways of cell death: oncosis, apoptosis, and necrosis. Toxicol. Path. 25: 82-88, 1997. [PubMed: 9061857, related citations] [Full Text]

  3. Zhang, C. H., Xu, Y. H., Gu, J. J., Schlossman, S. F. A cell surface receptor defined by mAb mediates a unique type of cell death similar to oncosis. Proc. Nat. Acad. Sci. 95: 6290-6295, 1998. [PubMed: 9600958, images, related citations] [Full Text]


Creation Date:
Victor A. McKusick : 10/5/2001
alopez : 07/18/2006
alopez : 3/19/2004
mcapotos : 12/20/2001
carol : 10/5/2001
carol : 10/5/2001

* 606356

TRANSMEMBRANE PROTEIN 123; TMEM123


Alternative titles; symbols

PRO-ONCOSIS RECEPTOR INDUCING MEMBRANE INJURY; PORIMIN
KERATINOCYTE-ASSOCIATED TRANSMEMBRANE PROTEIN 3; KCT3


HGNC Approved Gene Symbol: TMEM123

Cytogenetic location: 11q22.2     Genomic coordinates (GRCh38): 11:102,396,332-102,452,765 (from NCBI)


TEXT

Description

PORIMIN (pro-oncosis receptor inducing membrane injury), a member of the cell membrane-associated mucin family, mediates oncosis-like cell death.


Cloning and Expression

Zhang et al. (1998) reported the generation and characterization of a monoclonal antibody, designated anti-PORIMIN (pro-oncosis receptor inducing membrane injury), which mediated oncosis-like cell death in Jurkat cells. Anti-PORIMIN-triggered cell death showed no evidence of DNA fragmentation or formation of apoptotic bodies but rapidly showed plasma membrane injury followed by cell death. Ma et al. (2001) isolated PORIMIN cDNA from a Jurkat cell cDNA library by COS cell expression cloning. The cDNA encodes a deduced 189-amino acid type I transmembrane protein. Its extracellular domain contains many O-linked and 7 N-linked glycosylation sites that define it as a member of the mucin family. Northern blot analysis detected ubiquitous expression of a major 3.3-kb transcript, with comparable and relatively high levels in trachea, fetal kidney, uterus, thyroid gland, adult liver, and 2 tumor cell lines, colorectal adenocarcinoma SW480 and lung carcinoma A549. The only tissue that did not express PORIMIN was ovary. COS-7 and 293 cells transiently transfected with PORIMIN cDNA were specifically recognized by anti-PORIMIN antibody in cell staining and immunoblotting experiments.


Gene Structure

Ma et al. (2001) determined that the PORIMIN gene contains 4 exons and spans approximately 133 kb of genomic DNA.


Mapping

By sequence analysis, Ma et al. (2001) mapped the TMEM123 gene to chromosome 11q22.1.


History

The term oncosis, derived from the Greek 'onkos' (meaning swelling), was first used in 1910 to describe ischemic cell death in osteocytes and was later used by Trump et al. (1997) to describe a form of cell death distinct from apoptosis.


REFERENCES

  1. Ma, F., Zhang, C., Prasad, K. V. S., Freeman, G. J., Schlossman, S. F. Molecular cloning of porimin, a novel cell surface receptor mediating oncotic cell death. Proc. Nat. Acad. Sci. 98: 9778-9783, 2001. [PubMed: 11481458] [Full Text: https://doi.org/10.1073/pnas.171322898]

  2. Trump, B. F., Berezesky, I. K., Chang, S. H., Phelps, P. C. The pathways of cell death: oncosis, apoptosis, and necrosis. Toxicol. Path. 25: 82-88, 1997. [PubMed: 9061857] [Full Text: https://doi.org/10.1177/019262339702500116]

  3. Zhang, C. H., Xu, Y. H., Gu, J. J., Schlossman, S. F. A cell surface receptor defined by mAb mediates a unique type of cell death similar to oncosis. Proc. Nat. Acad. Sci. 95: 6290-6295, 1998. [PubMed: 9600958] [Full Text: https://doi.org/10.1073/pnas.95.11.6290]


Creation Date:
Victor A. McKusick : 10/5/2001

Edit History:
alopez : 07/18/2006
alopez : 3/19/2004
mcapotos : 12/20/2001
carol : 10/5/2001
carol : 10/5/2001