Alternative titles; symbols
HGNC Approved Gene Symbol: FBH1
Cytogenetic location: 10p15.1 Genomic coordinates (GRCh38): 10:5,889,572-5,937,593 (from NCBI)
DNA helicases are enzymes involved in DNA replication, repair, and recombination. They generate single-stranded DNA by catalyzing the melting of stable helical DNA structures utilizing energy derived from the hydrolysis of nucleoside triphosphates. FBH1 is a member of a unique class of DNA helicases that are also involved in cell cycle regulation by catalyzing ubiquitin ligation of proteins for ubiquitin-mediated proteolysis (Kim et al., 2002).
Kim et al. (2002) cloned FBH1 by searching for homologs of S. pombe DNA helicase I, screening a HeLa cell cDNA library, and 5-prime RACE. The deduced 969-amino acid protein contains an F-box motif and 7 conserved helicase motifs. It shares 95% identity with the mouse protein.
Kim et al. (2002) found that recombinant FBH1, purified from baculovirus-infected sf9 cells, showed elevated levels of single-stranded DNA-dependent ATPase activity. They determined that FBH1 translocates in the 3-prime-to-5-prime direction and is not dependent on fork structures for its unwinding activity. The presence of an F-box in the FBH1 sequence suggested its inclusion in SCF (SKP1 (601434)/cullin (see CUL1; 603134)/F-box) complexes, which are involved in ubiquitin-dependent proteolysis. Coimmunoprecipitation experiments with extracts of cotransfected human 293T cells confirmed that FBH1 interacts with SKP1 and, through SKP1, with CUL1 and ROC1 (RBX1; 603814) in vitro. All interactions were dependent on a functional F-box motif. The SCF complex including FBH1 displayed ubiquitin ligase activity.
Jin et al. (2004) stated that the FBXO18 gene maps to chromosome 10p15.1 and the mouse Fbxo18 gene maps to chromosome 2A1.
Jin, J., Cardozo, T., Lovering, R. C., Elledge, S. J., Pagano, M., Harper, J. W. Systematic analysis and nomenclature of mammalian F-box proteins. Genes Dev. 18: 2573-2580, 2004. [PubMed: 15520277] [Full Text: https://doi.org/10.1101/gad.1255304]
Kim, J., Kim, J.-H., Lee, S.-H., Kim, D.-H., Kang, H.-Y., Bae, S.-H., Pan, Z.-Q., Seo, Y.-S. The novel human DNA helicase hFBH1 is an F-box protein. J. Biol. Chem. 277: 24530-24537, 2002. [PubMed: 11956208] [Full Text: https://doi.org/10.1074/jbc.M201612200]