Alternative titles; symbols
HGNC Approved Gene Symbol: NUP58
Cytogenetic location: 13q12.13 Genomic coordinates (GRCh38): 13:25,301,625-25,349,800 (from NCBI)
By sequencing clones obtained from a size-fractionated brain cDNA library, Ishikawa et al. (1997) cloned NUPL1, which they designated KIAA0410. The deduced 485-amino acid protein has a calculated molecular mass of about 53 kD. NUPL1 shares 87% sequence identity with rat nucleoporin p58. RT-PCR detected low-level NUPL1 expression in all tissues examined.
Crystal Structure
Chug et al. (2015) reported a structural analysis of the Nup62 (605815)-Nup58-Nup54 (607607) complex, which is a crucial component of the nucleocytoplasmic transport system. It comprises an approximately 13 nanometer-long trimerization interface with an unusual 2W3F coil, a canonical heterotrimeric coiled coil, and a kink that enforces a compact 6-helix bundle. Nup54 also contains a ferredoxin (103260)-like domain. Chug et al. (2015) further identified a heterotrimeric Nup93 (614351)-binding module for nuclear pore complex (NPC) anchorage. The quaternary structure alternations in the Nup62 complex, which had been proposed to trigger a general gating of the NPC, are incompatible with the trimer structure. Chug et al. (2015) suggested that the highly elongated Nup62 complex projects barrier-forming FG (phe-gly) repeats far into the central NPC channel, supporting a barrier that guards the entire cross-section.
By radiation hybrid analysis, Ishikawa et al. (1997) mapped the NUP58 gene to chromosome 13.
Chug, H., Trakhanov, S., Hulsmann, B. B., Pleiner, T., Gorlich, D. Crystal structure of the metazoan Nup62-Nup58-Nup54 nucleoporin complex. Science 350: 106-110, 2015. [PubMed: 26292704] [Full Text: https://doi.org/10.1126/science.aac7420]
Ishikawa, K., Nagase, T., Nakajima, D., Seki, N., Ohira, M., Miyajima, N., Tanaka, A., Kotani, H., Nomura, N., Ohara, O. Prediction of the coding sequences of unidentified human genes. VIII. 78 new cDNA clones from brain which code for large proteins in vitro. DNA Res. 4: 307-313, 1997. [PubMed: 9455477] [Full Text: https://doi.org/10.1093/dnares/4.5.307]