Alternative titles; symbols
HGNC Approved Gene Symbol: ECI2
Cytogenetic location: 6p25.2 Genomic coordinates (GRCh38): 6:4,115,706-4,135,575 (from NCBI)
PECI is an auxiliary enzyme that catalyzes an isomerization step required for the beta-oxidation of unsaturated fatty acids.
By searching databases for sequences similar to yeast Eci1, followed by 5-prime RACE of a fetal liver cDNA library, Geisbrecht et al. (1999) cloned PECI. The deduced 359-amino acid protein has a calculated molecular mass of 39.6 kD. They also cloned mouse Peci, which encodes a deduced 358-amino acid protein with a calculated molecular mass of 39.4 kD. Human PECI contains 2 putative start codons, while mouse Peci contains only the second start codon. Both contain a C-terminal peroxisomal targeting signal. Northern blot analysis detected a 1.5-kb transcript in all human tissues examined. Highest expression was detected in heart, skeletal muscle, and liver. Western blot analysis of fibroblasts showed that endogenous PECI migrated with an apparent molecular mass of 39 kD. PECI partitioned with peroxisomal markers following fractionation of hepatocellular carcinoma cells, and immunofluorescence microscopy of differentially permeabilized fibroblasts indicated that PECI is a peroxisomal matrix protein.
Using pooled antisera from autoimmune diabetes patients to screen a pancreatic islet cell cDNA library, Suk et al. (1999) cloned PECI, which they called DRS1. The deduced protein contains 364 amino acids. Northern blot analysis detected a 1.4-kb transcript in all tissues examined, with highest level in liver and lowest levels in brain and muscle. RT-PCR detected expression in HeLa cells, Jurkat T cells, and isolated pancreatic islet cells. In vitro transcription and translation within a reticulocyte lysate system resulted in 2 major proteins with apparent molecular masses of 36 and 40 kD.
Geisbrecht et al. (1999) determined that recombinant PECI, expressed in E. coli, catalyzed the isomerization of 3-cis-octenoyl-CoA to 2-trans-octenoyl-CoA.
The International Radiation Hybrid Mapping Consortium mapped the PECI gene to chromosome 6 (stSG6358).
Geisbrecht, B. V., Zhang, D., Schulz, H., Gould, S. J. Characterization of PECI, a novel monofunctional delta-3,delta-2-enoyl-CoA isomerase of mammalian peroxisomes. J. Biol. Chem. 274: 21797-21803, 1999. [PubMed: 10419495] [Full Text: https://doi.org/10.1074/jbc.274.31.21797]
Suk, K., Kim, Y.-H., Hwang, D.-Y., Ihm, S.-H., Yoo, H. J., Lee, M.-S. Molecular cloning and expression of a novel human cDNA related to the diazepam binding inhibitor. Biochim. Biophys. Acta 1454: 126-131, 1999. [PubMed: 10354522] [Full Text: https://doi.org/10.1016/s0925-4439(99)00033-2]