Alternative titles; symbols
HGNC Approved Gene Symbol: ASF1B
Cytogenetic location: 19p13.12 Genomic coordinates (GRCh38): 19:14,119,512-14,136,589 (from NCBI)
Using TLK1 (608438) and TLK2 (608439) as bait in a yeast 2-hybrid screen of a peripheral lymphocyte cDNA library, Sillje and Nigg (2001) cloned ASF1B and ASF1A (609189). The deduced 203-amino acid ASF1B protein has a calculated molecular mass of 22.4 kD. ASF1B and ASF1A share 71% amino acid identity, and they share about 50% identity with yeast Asf1, which has a C-terminal acidic stretch not found in the human ASF1 proteins.
Sillje and Nigg (2001) found that in vitro translated ASF1A and ASF1B were phosphorylated by TLK1 and TLK2. The levels of ASF1A and ASF1B were unchanged during the cell cycle in synchronized HeLa cells.
Mello et al. (2002) found that ASF1A and ASF1B interacted directly and functioned synergistically with human CAF1 (see 601245) to assemble nucleosomes during nucleotide excision repair in vitro.
Groth et al. (2007) characterized the complex in which the human histone chaperone ASF1 and MCM2-7 (see 116945), the putative replicative helicase, are connected through a histone H3-H4 bridge. Depletion of ASF1 by RNA interference impeded DNA unwinding at replication sites, and similar defects arose from overproduction of new histone H3-H4 that compromised ASF1 function. Groth et al. (2007) concluded that their data link ASF1 chaperone function, histone supply, and replicative unwinding of DNA in chromatin. Groth et al. (2007) proposed that ASF1, as a histone acceptor and donor, handles parental and new histones at the replication fork via an ASF1-(H3-H4)-MCM2-7 intermediate and thus provides a means to fine-tune replication fork progression and histone supply and demand.
Hartz (2005) mapped the ASF1B gene to chromosome 19p13.13 based on an alignment of the ASF1B sequence (GenBank AB104486) with the genomic sequence.
Groth, A., Corpet, A., Cook, A. J. L., Roche, D., Bartek, J., Lukas, J., Almouzni, G. Regulation of replication fork progression through histone supply and demand. Science 318: 1928-1931, 2007. [PubMed: 18096807] [Full Text: https://doi.org/10.1126/science.1148992]
Hartz, P. A. Personal Communication. Baltimore, Md. 2/3/2005.
Mello, J. A., Sillje, H. H. W., Roche, D. M. J., Kirschner, D. B., Nigg, E. A., Almouzni, G. Human Asf1 and CAF-1 interact and synergize in a repair-coupled nucleosome assembly pathway. EMBO Rep. 3: 329-334, 2002. [PubMed: 11897662] [Full Text: https://doi.org/10.1093/embo-reports/kvf068]
Sillje, H. H. W., Nigg, E. A. Identification of human Asf1 chromatin assembly factors as substrates of Tousled-like kinases. Curr. Biol. 11: 1068-1073, 2001. [PubMed: 11470414] [Full Text: https://doi.org/10.1016/s0960-9822(01)00298-6]