Alternative titles; symbols
HGNC Approved Gene Symbol: CPXM1
Cytogenetic location: 20p13 Genomic coordinates (GRCh38): 20:2,794,074-2,800,627 (from NCBI)
By searching an EST database for sequences similar to human CPE (114855), followed by 5-prime RACE, Lei et al. (1999) cloned mouse Cpxm, which they called Cpx1. The deduced 722-amino acid protein has a calculated molecular mass of 79 kD. Cpx1 contains an N-terminal discoidin domain and a C-terminal carboxypeptidase domain that includes a putative calcium-binding region but lacks several residues important for catalytic activity in other carboxypeptidases. Northern blot analysis detected a 2.5-kb transcript in most tissues examined, with highest levels in testis and spleen, moderate levels in salivary gland, brain, heart, lung, and kidney, and low levels in liver and muscle. No expression was detected in eye, adrenal, and white adipose tissue.
Lei et al. (1999) found that mouse Cpx1 expressed either in insect cells or in a mouse cell line did not cleave standard carboxypeptidase substrates.
Deloukas et al. (2001) mapped the CPXM gene to chromosome 20p near the D20S198 marker.
Deloukas, P., Matthews, L. H., Ashurst, J., Burton, J., Gilbert, J. G. R., Jones, M., Stavrides, G., Almeida, J. P., Babbage, A. K., Bagguley, C. L., Bailey, J., Barlow, K. F., and 115 others. The DNA sequence and comparative analysis of human chromosome 20. Nature 414: 865-871, 2001. [PubMed: 11780052] [Full Text: https://doi.org/10.1038/414865a]
Lei, Y., Xin, X., Morgan, D., Pintar, J. E., Fricker, L. D. Identification of mouse CPX-1, a novel member of the metallocarboxypeptidase gene family with highest similarity to CPX-2. DNA Cell Biol. 18: 175-185, 1999. [PubMed: 10073577] [Full Text: https://doi.org/10.1089/104454999315565]