Entry - *610625 - ADP-RIBOSYLTRANSFERASE 5; ART5 - OMIM

 
 
* 610625

ADP-RIBOSYLTRANSFERASE 5; ART5


HGNC Approved Gene Symbol: ART5

Cytogenetic location: 11p15.4     Genomic coordinates (GRCh38): 11:3,638,512-3,642,379 (from NCBI)


TEXT

▼ Description

ADP-ribosylation is a reversible posttranslational modification used to regulate protein function. ADP-ribosyltransferases, such as ART5, transfer ADP-ribose from NAD+ to the target protein, and ADP-ribosylhydrolases (see PARG; 603501) reverse the reaction (Glowacki et al., 2002).


▼ Cloning and Expression

By PCR using degenerate primers corresponding to conserved regions of mammalian ARTs, Glowacki et al. (2002) cloned mouse and human ART5. The deduced protein contains a hydrophobic N-terminal peptide, an R-S-EXE motif characteristic of arginine-specific ARTs, and 4 conserved cysteines. Northern blot analysis and RT-PCR detected predominant ART5 expression in mouse heart, skeletal muscle, and testis, and in human testis.

Glowacki et al. (2001) identified 6 splice variants of mouse Art5. The full-length mouse protein is 87% identical to its human homolog. Unlike other Art proteins, Art5 lacks a glycosylphosphatidylinositol anchor signal sequence and is a secretory enzyme.


▼ Gene Function

Glowacki et al. (2002) found that ART5 had potent arginine-specific ADP-ribosylation activity following in vitro translation.


▼ Gene Structure

Glowacki et al. (2002) determined that the ART5 gene spans 3.8 kb and contains 5 exons, the first of which is untranslated.


▼ Mapping

By genomic sequence analysis, Glowacki et al. (2002) mapped the ART5 gene to chromosome 11p15. Glowacki et al. (2001) mapped the mouse Art5 gene to a region of chromosome 7 containing other Art genes.


▼ REFERENCES

  1. Glowacki, G., Braren, R., Cetkovic-Cvrlje, M., Leiter, E. H., Haag, F., Koch-Nolte, F. Structure, chromosomal localization, and expression of the gene for mouse ecto-mono(ADP-ribosyl)transferase ART5. Gene 275: 267-277, 2001. [PubMed: 11587854, related citations] [Full Text]

  2. Glowacki, G., Braren, R., Firner, K., Nissen, M., Kuhl, M., Reche, P., Bazan, F., Cetkovic-Cvrlje, M., Leiter, E., Haag, F., Koch-Nolte, F. The family of toxin-related ecto-ADP-ribosyltransferases in humans and the mouse. Protein Sci. 11: 1657-1670, 2002. [PubMed: 12070318, images, related citations] [Full Text]


Creation Date:
Patricia A. Hartz : 11/30/2006
Edit History:
mgross : 11/30/2006

* 610625

ADP-RIBOSYLTRANSFERASE 5; ART5


HGNC Approved Gene Symbol: ART5

Cytogenetic location: 11p15.4     Genomic coordinates (GRCh38): 11:3,638,512-3,642,379 (from NCBI)


TEXT

Description

ADP-ribosylation is a reversible posttranslational modification used to regulate protein function. ADP-ribosyltransferases, such as ART5, transfer ADP-ribose from NAD+ to the target protein, and ADP-ribosylhydrolases (see PARG; 603501) reverse the reaction (Glowacki et al., 2002).


Cloning and Expression

By PCR using degenerate primers corresponding to conserved regions of mammalian ARTs, Glowacki et al. (2002) cloned mouse and human ART5. The deduced protein contains a hydrophobic N-terminal peptide, an R-S-EXE motif characteristic of arginine-specific ARTs, and 4 conserved cysteines. Northern blot analysis and RT-PCR detected predominant ART5 expression in mouse heart, skeletal muscle, and testis, and in human testis.

Glowacki et al. (2001) identified 6 splice variants of mouse Art5. The full-length mouse protein is 87% identical to its human homolog. Unlike other Art proteins, Art5 lacks a glycosylphosphatidylinositol anchor signal sequence and is a secretory enzyme.


Gene Function

Glowacki et al. (2002) found that ART5 had potent arginine-specific ADP-ribosylation activity following in vitro translation.


Gene Structure

Glowacki et al. (2002) determined that the ART5 gene spans 3.8 kb and contains 5 exons, the first of which is untranslated.


Mapping

By genomic sequence analysis, Glowacki et al. (2002) mapped the ART5 gene to chromosome 11p15. Glowacki et al. (2001) mapped the mouse Art5 gene to a region of chromosome 7 containing other Art genes.


REFERENCES

  1. Glowacki, G., Braren, R., Cetkovic-Cvrlje, M., Leiter, E. H., Haag, F., Koch-Nolte, F. Structure, chromosomal localization, and expression of the gene for mouse ecto-mono(ADP-ribosyl)transferase ART5. Gene 275: 267-277, 2001. [PubMed: 11587854] [Full Text: https://doi.org/10.1016/s0378-1119(01)00608-4]

  2. Glowacki, G., Braren, R., Firner, K., Nissen, M., Kuhl, M., Reche, P., Bazan, F., Cetkovic-Cvrlje, M., Leiter, E., Haag, F., Koch-Nolte, F. The family of toxin-related ecto-ADP-ribosyltransferases in humans and the mouse. Protein Sci. 11: 1657-1670, 2002. [PubMed: 12070318] [Full Text: https://doi.org/10.1110/ps.0200602]


Creation Date:
Patricia A. Hartz : 11/30/2006

Edit History:
mgross : 11/30/2006



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OMIM® and Online Mendelian Inheritance in Man® are registered trademarks of the Johns Hopkins University.
Copyright® 1966-2024 Johns Hopkins University.

NOTE: OMIM is intended for use primarily by physicians and other professionals concerned with genetic disorders, by genetics researchers, and by advanced students in science and medicine. While the OMIM database is open to the public, users seeking information about a personal medical or genetic condition are urged to consult with a qualified physician for diagnosis and for answers to personal questions.
OMIM® and Online Mendelian Inheritance in Man® are registered trademarks of the Johns Hopkins University.
Copyright® 1966-2024 Johns Hopkins University.
Printed: May 17, 2024