Alternative titles; symbols
HGNC Approved Gene Symbol: NUBP2
Cytogenetic location: 16p13.3 Genomic coordinates (GRCh38): 16:1,782,960-1,789,186 (from NCBI)
NUBP2 is a member of the NUBP/MRP gene subfamily of ATP-binding proteins (see NUBP1; 600280) (Nakashima et al., 1999).
Nakashima et al. (1999) cloned mouse Nubp2 and used the murine sequence as a probe in EST database analysis, followed by screening a human teratoma cDNA library to clone full-length human NUBP2. The 271-amino acid human NUBP2 protein shares 72.6% amino acid similarity with mouse Nubp2. Northern blot analysis detected a 1.4-kb NUBP2 transcript with ubiquitous expression in all human adult and fetal tissues tested, with highest expression in adult skeletal muscle. NUBP2 contains conserved an ATP/GTP binding motif A (P-loop), an ATP/GTP binding motif A-prime, and NUBP/MRP alpha and beta motifs. NUBP2 lacks an additional N-terminal sequence with 4 cysteine residues that is present in NUBP1.
Roy et al. (2003) showed that yeast Cfd1 was required for cytosolic Fe-S cluster biogenesis and conversion of mammalian IRP1 (ACO1; 100880) to cytosolic aconitase in yeast.
Stehling et al. (2008) found that NBP35 (NUBP1) interacted with coexpressed CFD1 in transfected HeLa cells, suggesting that, like NBP35, CFD1 may function in the regulation of cellular iron homeostasis and cytosolic Fe-S protein assembly.
Hartz (2012) mapped the NUBP2 gene to chromosome 16p13.3 based on an alignment of the NUBP2 sequence (GenBank AF118394) with the genomic sequence (GRCh37).
Nakashima et al. (1999) mapped the mouse Nubp2 gene to the t-complex region of chromosome 17, which shows homology of synteny to human chromosome 16p13.3.
Hartz, P. A. Personal Communication. Baltimore, Md. 9/11/2012.
Nakashima, H., Grahovac, M. J., Mazzarella, R., Fujiwara, H., Kitchen, J. R., Threat, T. A., Ko, M. S. H. Two novel mouse genes--Nubp2, mapped to the t-complex on chromosome 17, and Nubp1, mapped to the chromosome 16--establish a new gene family of nucleotide-binding proteins in eukaryotes. Genomics 60: 152-160, 1999. [PubMed: 10486206] [Full Text: https://doi.org/10.1006/geno.1999.5898]
Roy, A., Solodovnikova, N., Nicholson, T., Antholine, W., Walden, W. E. A novel eukaryotic factor for cytosolic Fe-S cluster assembly. EMBO J. 22: 4826-4835, 2003. [PubMed: 12970194] [Full Text: https://doi.org/10.1093/emboj/cdg455]
Stehling, O., Netz, D. J. A., Niggemeyer, B., Rosser, R., Eisenstein, R. S., Puccio, H., Pierik, A. J., Lill, R. Human Nbp35 is essential for both cytosolic iron-sulfur protein assembly and iron homeostasis. Molec. Cell. Biol. 28: 5517-5528, 2008. [PubMed: 18573874] [Full Text: https://doi.org/10.1128/MCB.00545-08]