Alternative titles; symbols
HGNC Approved Gene Symbol: UTP3
Cytogenetic location: 4q13.3 Genomic coordinates (GRCh38): 4:70,688,532-70,690,551 (from NCBI)
Sakuma et al. (2001) cloned mouse Utp3, which they called Crl1. The deduced 469-amino acid protein is rich in charged amino acids and contains a putative leucine zipper region and a region that shares significant homology with yeast Sas10. In situ hybridization revealed expression in olfactory bulb and cerebral cortex in mouse embryos at 17.5 days postcoitum. Postnatally, Crl1 expression was also observed in cerebellar cortex, with strong expression in hippocampus.
By yeast 2-hybrid analysis, Sakuma et al. (2001) showed that mouse Crl1 interacted specifically with Pebp2b2, which is encoded by a splice variant of Pebp2b (CBFB; 121360).
Lim et al. (2006) showed that the CRLZ1 and IgJ (147790) genes are adjacent but divergently transcribed in human and mouse. By chromatin immunoprecipitation and FACS analysis of sorted mouse pre-B cells and plasma cells, they found that IgJ was expressed in the plasma cell stage, whereas Crlz1 was expressed in the pre-B cell stage. The stage-specific expression of IgJ and Crlz1 was regulated by chromatin accessibility and acetylation. DNase I hypersensitive site 1 on the IgJ promoter was opened in plasma cells, but hypersensitive sites 9 and 10 on the Crlz1 promoter were opened in pre-B cells. H3 (see 602810) and H4 (see 602822) histones were hyperacetylated in the chromatin of Crlz1 in pre-B cells, whereas those in the chromatin of IgJ were hyperacetylated in plasma cells. Lim et al. (2006) concluded that the CRLZ1-IgJ locus shows stage-specific gene expression regulation, and they proposed that additional regulatory elements may exist between the genes to coordinate chromatin accessibility and histone acetylation over the locus.
By genomic sequence analysis, Lim et al. (2006) mapped the UTP3 gene upstream of the IGJ gene on chromosome 4q21. The UTP3 and IGJ genes are transcribed in opposite directions, and the positioning and orientation of the genes are conserved on mouse chromosome 5.
Lim, J.-H., Cho, S.-J., Park, S.-K., Kim, J., Cho, D., Lee, W. J., Kang, C.-J. Stage-specific expression of two neighboring Crlz1 and IgJ genes during B cell development is regulated by their chromatin accessibility and histone acetylation. J. Immun. 177: 5420-5429, 2006. [PubMed: 17015728] [Full Text: https://doi.org/10.4049/jimmunol.177.8.5420]
Sakuma, T., Li, Q.-L., Jin, Y., Choi, L.-W., Kim, E.-G., Ito, K., Ito, Y., Nomura, S., Bae, S.-C. Cloning and expression pattern of a novel PEBP2-beta-binding protein (charged amino acid rich leucine zipper-1 [Crl-1]) in the mouse. Mech. Dev. 104: 151-154, 2001. [PubMed: 11404095] [Full Text: https://doi.org/10.1016/s0925-4773(01)00366-5]