Entry - *612427 - RNA-BINDING MOTIF PROTEIN 25; RBM25 - OMIM

 
 
* 612427

RNA-BINDING MOTIF PROTEIN 25; RBM25


Alternative titles; symbols

ARG/GLU/ASP-RICH PROTEIN, 120-KD; RED120


HGNC Approved Gene Symbol: RBM25

Cytogenetic location: 14q24.2     Genomic coordinates (GRCh38): 14:73,058,534-73,123,899 (from NCBI)


TEXT

▼ Cloning and Expression

Using mass spectrometric analysis to identify proteins associated with the splicing coactivators SRM160 (SRRM1; 605975) and SRM300 (SRRM2; 606032) in HeLa cell nuclear lysates, followed by database analysis, Fortes et al. (2007) identified RBM25, which they called RED120. The deduced 843-amino acid protein has a calculated molecular mass of 120 kD. It contains an N-terminal proline-rich domain, followed by an RNA recognition motif, a region rich in RE/RD dipeptides, and a C-terminal PWI domain predicted to be involved in RNA binding and 3-prime end processing. Immunohistochemical analysis revealed that RED120 was concentrated in interchromatin granules, or nuclear speckles, and additional staining was detected in the cytoplasm.


▼ Gene Function

Using reciprocal immunoprecipitation analysis of HeLa cell nuclear lysates, Fortes et al. (2007) confirmed a direct interaction between RED120 and SRM160. The RED120-SRM160 interaction was not sensitive to RNase treatment. RED120 associated with multiple splicing components, including CBP80 (NCBP1; 600469), Sm proteins (see 603451), and small nuclear RNAs (see 180680). RNase treatment abolished the interaction between RED120 and CBP80, but not the interactions between RED120 and other splicing components. Immunodepletion of RED120 did not prevent splicing or cleavage of 4 different pre-mRNAs. RED120 maintained its association with mRNA after splicing, and it associated with an mRNA-derived exon product following splicing more efficiently than a cDNA-derived exon.


▼ Mapping

Fortes et al. (2007) stated that the RBM25 gene maps to chromosome 14. Hartz (2008) mapped the RBP25 gene to chromosome 14q24.2 based on an alignment of the RBM25 sequence (GenBank AK026107) with the genomic sequence (build 36.1).


▼ REFERENCES

  1. Fortes, P., Longman, D., McCracken, S., Ip, J. Y., Poot, R., Mattaj, I. W., Caceres, J. F., Blencowe, B. J. Identification and characterization of RED120: a conserved PWI domain protein with links to splicing and 3-prime-end formation. FEBS Lett. 581: 3087-3097, 2007. [PubMed: 17560998, related citations] [Full Text]

  2. Hartz, P. A. Personal Communication. Baltimore, Md. 11/20/2008.


Creation Date:
Patricia A. Hartz : 11/20/2008
Edit History:
mgross : 11/20/2008

* 612427

RNA-BINDING MOTIF PROTEIN 25; RBM25


Alternative titles; symbols

ARG/GLU/ASP-RICH PROTEIN, 120-KD; RED120


HGNC Approved Gene Symbol: RBM25

Cytogenetic location: 14q24.2     Genomic coordinates (GRCh38): 14:73,058,534-73,123,899 (from NCBI)


TEXT

Cloning and Expression

Using mass spectrometric analysis to identify proteins associated with the splicing coactivators SRM160 (SRRM1; 605975) and SRM300 (SRRM2; 606032) in HeLa cell nuclear lysates, followed by database analysis, Fortes et al. (2007) identified RBM25, which they called RED120. The deduced 843-amino acid protein has a calculated molecular mass of 120 kD. It contains an N-terminal proline-rich domain, followed by an RNA recognition motif, a region rich in RE/RD dipeptides, and a C-terminal PWI domain predicted to be involved in RNA binding and 3-prime end processing. Immunohistochemical analysis revealed that RED120 was concentrated in interchromatin granules, or nuclear speckles, and additional staining was detected in the cytoplasm.


Gene Function

Using reciprocal immunoprecipitation analysis of HeLa cell nuclear lysates, Fortes et al. (2007) confirmed a direct interaction between RED120 and SRM160. The RED120-SRM160 interaction was not sensitive to RNase treatment. RED120 associated with multiple splicing components, including CBP80 (NCBP1; 600469), Sm proteins (see 603451), and small nuclear RNAs (see 180680). RNase treatment abolished the interaction between RED120 and CBP80, but not the interactions between RED120 and other splicing components. Immunodepletion of RED120 did not prevent splicing or cleavage of 4 different pre-mRNAs. RED120 maintained its association with mRNA after splicing, and it associated with an mRNA-derived exon product following splicing more efficiently than a cDNA-derived exon.


Mapping

Fortes et al. (2007) stated that the RBM25 gene maps to chromosome 14. Hartz (2008) mapped the RBP25 gene to chromosome 14q24.2 based on an alignment of the RBM25 sequence (GenBank AK026107) with the genomic sequence (build 36.1).


REFERENCES

  1. Fortes, P., Longman, D., McCracken, S., Ip, J. Y., Poot, R., Mattaj, I. W., Caceres, J. F., Blencowe, B. J. Identification and characterization of RED120: a conserved PWI domain protein with links to splicing and 3-prime-end formation. FEBS Lett. 581: 3087-3097, 2007. [PubMed: 17560998] [Full Text: https://doi.org/10.1016/j.febslet.2007.05.066]

  2. Hartz, P. A. Personal Communication. Baltimore, Md. 11/20/2008.


Creation Date:
Patricia A. Hartz : 11/20/2008

Edit History:
mgross : 11/20/2008



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OMIM® and Online Mendelian Inheritance in Man® are registered trademarks of the Johns Hopkins University.
Copyright® 1966-2024 Johns Hopkins University.

NOTE: OMIM is intended for use primarily by physicians and other professionals concerned with genetic disorders, by genetics researchers, and by advanced students in science and medicine. While the OMIM database is open to the public, users seeking information about a personal medical or genetic condition are urged to consult with a qualified physician for diagnosis and for answers to personal questions.
OMIM® and Online Mendelian Inheritance in Man® are registered trademarks of the Johns Hopkins University.
Copyright® 1966-2024 Johns Hopkins University.
Printed: June 4, 2024