Alternative titles; symbols
HGNC Approved Gene Symbol: KLHL6
Cytogenetic location: 3q27.1 Genomic coordinates (GRCh38): 3:183,487,551-183,555,706 (from NCBI)
By sequencing clones obtained from a size-fractionated human spleen cDNA library, Hattori et al. (2000) isolated a partial KLHL6 clone, which they designated FLJ00029. RT-PCR ELISA detected highest expression in spleen, with lower expression in lung, liver, kidney, and ovary. Weak expression was detected in heart, brain, and pancreas, and little to no expression was detected in skeletal muscle and testis.
By searching an EST database for sequences similar to ovine Klhl6, followed by 5-prime RACE of a Burkitt lymphoma cDNA library and screening of a Daudi cell cDNA library, Gupta-Rossi et al. (2003) cloned KLHL6. The deduced 610-amino acid protein contains an N-terminal BTB/POZ domain and 5 tandem C-terminal Kelch repeats. Northern blot analysis detected a KLHL6 transcript of about 7.5 kb that was expressed predominantly in germinal center B cells. Lower expression was detected in tonsil and thymus, and no expression was detected in non-lymphoid tissues. A 9-kb transcript was also detected in germinal center B cells.
Using subtractive hybridization, Gupta-Rossi et al. (2003) found that expression of Klhl6 was upregulated in sheep ileal Peyer patch follicular B cells in concert with immunoglobulin gene hypermutation.
Gupta-Rossi et al. (2003) found that the 5-prime end of the KLHL6 gene contains an ALU element. The 3-prime noncoding region, which is highly conserved between sheep and human, covers 3.6 kb.
Hartz (2011) mapped the KLHL6 gene to chromosome 3q27.3 based on an alignment of the KLHL6 sequence (GenBank AK024439) with the genomic sequence (GRCh37).
Gupta-Rossi, N., Storck, S., Griebel, P. J., Reynaud, C.-A., Weill, J.-C., Dahan, A. Specific over-expression of deltex and a new Kelch-like protein in human germinal center B cells. Molec. Immun. 39: 791-799, 2003. [PubMed: 12617994] [Full Text: https://doi.org/10.1016/s0161-5890(03)00002-6]
Hartz, P. A. Personal Communication. Baltimore, Md. 9/6/2011.
Hattori, A., Okumura, K., Nagase, T., Kikuno, R., Hirosawa, M., Ohara, O. Characterization of long cDNA clones from human adult spleen. DNA Res. 7: 357-366, 2000. [PubMed: 11214971] [Full Text: https://doi.org/10.1093/dnares/7.6.357]