HGNC Approved Gene Symbol: DYDC1
Cytogenetic location: 10q23.1 Genomic coordinates (GRCh38): 10:80,336,013-80,356,755 (from NCBI)
Based on experiments in mice, DYDC1 is predicted to have a role in spermatogenesis and acrosome formation (Li et al., 2009).
Using the N-terminal BAR domain of SH3P13 (SH3GL3; 603362) as bait in a yeast 2-hybrid screen of a human testis cDNA library, Li et al. (2009) cloned DYDC1. Northern blot analysis of 11 human tissues detected DYDC1 expression in testis and brain only. Immunohistochemical analysis of normal mouse testis showed accumulation of Dydc1 in the acrosome region during spermiogenesis.
Using immunoprecipitation analysis and protein pull-down assays, Li et al. (2009) confirmed that SH3P13 interacted with DYDC1. The mouse GC-1 spg spermatogonia cell line differentiated normally following injection into the rete cavity of recipient mouse testis. However, knockdown of Dydc1 in GC-1 spg cells blocked spermatogenesis and acrosome formation. Similarly, injection of anti-Dydc1 antiserum into mouse seminiferous tubules blocked acrosome biogenesis.
Hartz (2013) mapped the DYDC1 gene to chromosome 10q23.1 based on an alignment of the DYDC1 sequence (GenBank BC019250) with the genomic sequence (GRCh37).
Hartz, P. A. Personal Communication. Baltimore, Md. 3/22/2013.
Li, S., Qiao, Y., Di, Q., Le, X., Zhang, L., Zhang, X., Zhang, C., Cheng, J., Zong, S., Koide, S. S., Miao, S., Wang, L. Interaction of SH3P13 and DYDC1 protein: a germ cell component that regulates acrosome biogenesis during spermiogenesis. Europ. J. Cell Biol. 88: 509-520, 2009. Note: Erratum: Europ. J. Cell Biol. 98: 51-52, 2019. [PubMed: 19545932] [Full Text: https://doi.org/10.1016/j.ejcb.2009.05.001]