* 615998

RING FINGER PROTEIN 10; RNF10


Alternative titles; symbols

KIAA0262


HGNC Approved Gene Symbol: RNF10

Cytogenetic location: 12q24.31     Genomic coordinates (GRCh38): 12:120,534,356-120,577,588 (from NCBI)


TEXT

Description

Proteins with RING finger domains, such as RNF10, have roles in development, transcriptional regulation, signal transduction, DNA repair, apoptosis, and oncogenesis (Hoshikawa et al., 2008).


Cloning and Expression

By sequencing clones obtained from a size-fractionated KG-1 immature myeloid cell line cDNA library, Nagase et al. (1996) obtained a partial RNF10 clone, which they designated KIAA0262. Northern blot analysis detected variable expression of 2 RNF10 transcripts in all adult tissues and cell lines examined. Highest expression was detected in heart and skeletal muscle.

By searching an EST database for sequences similar to mouse Rnf10, followed by 5-prime and 3-prime RACE analysis of a fetal brain cDNA library, Seki et al. (2000) cloned human RNF10. The deduced 811-amino acid human protein shares 91% identity with the 804-amino acid mouse protein. Both mouse and human RNF10 have an N-terminal zinc-binding C3HC4-type RING finger domain followed by 3 nuclear localization signals. RT-PCR detected Rnf10 expression in all adult and fetal mouse tissues examined.


Gene Function

Using protein interaction assays, Lin et al. (2005) found that RNF10 interacted with MEOX2 (600535), a homeobox transcription factor that regulates proliferation, differentiation, and migration of vascular smooth muscle cells and cardiomyocytes. Domain analysis revealed that the C-terminal region of RNF10 interacted with a central region of MEOX2 between the histidine/glutamine-rich region and the homeodomain. RNF10 and MEOX2 individually activated a p21(WAF1) (CDKN1A; 116899) reporter in transfected NIH-3T3 cells, and together they showed synergistic activation of p21(WAF1).

Myelin-associated glycoprotein (MAG; 159460) is detected in Schwann cells prior to the onset of myelination, suggesting that MAG initiates myelination. Using a Schwann cell-specific element (SSE) in the promoter region of rat Mag as bait, Hoshikawa et al. (2008) identified an interaction between Mag and Rnf10 in rat Schwann cells. EMSA and chromatin immunoprecipitation assays confirmed the interaction of Rnf10 with the SSE of Mag. Overexpression of Rnf10 in Schwann cells increased Mag promoter activity, and activation of the Mag promoter required the RING finger domain of Rnf10. Knockdown of Rnf10 in cultured Schwann cells decreased Mag expression, increased Schwann cell proliferation, and reduced myelination of neurons in Schwann cell-neuron cocultures. Rnf10 showed lower activation of the Mag reporter when expressed in rat osteosarcoma cells, suggesting that Rnf10 requires a Schwann cell-specific partner for maximal Mag activation.


Gene Structure

Seki et al. (2000) determined that the RNF10 gene contains 17 exons and spans approximately 40 kb.


Mapping

Using radiation hybrid analysis, Nagase et al. (1996) mapped the RNF10 gene to chromosome 12.

Hartz (2014) mapped the RNF10 gene to chromosome 12q24.31 based on an alignment of the RNF10 sequence (GenBank D87451) with the genomic sequence (GRCh38).

By radiation hybrid analysis, Seki et al. (2000) mapped the mouse Rnf10 gene to a region of chromosome 5 that shares homology of synteny with human chromosome 12q23-q24.


REFERENCES

  1. Hartz, P. A. Personal Communication. Baltimore, Md. 9/5/2014.

  2. Hoshikawa, S., Ogata, T., Fujiwara, S., Nakamura, K., Tanaka, S. A novel function of RING finger protein 10 in transcriptional regulation of the myelin-associated glycoprotein gene and myelin formation in Schwann cells. PLoS One 3: e3464, 2008. Note: Electronic Article. [PubMed: 18941509, images, related citations] [Full Text]

  3. Lin, J., Friesen, M. T., Bocangel, P., Cheung, D., Rawszer, K., Wigle, J. T. Characterization of mesenchyme homeobox 2 (MEOX2) transcription factor binding to RING finger protein 10. Molec. Cell. Biochem. 275: 75-84, 2005. [PubMed: 16335786, related citations] [Full Text]

  4. Nagase, T., Seki, N., Ishikawa, K., Ohira, M., Kawarabayasi, Y., Ohara, O., Tanaka, A., Kotani, H., Miyajima, N., Nomura, N. Prediction of the coding sequences of unidentified human genes. VI. The coding sequences of 80 new genes (KIAA0201-KIAA0280) deduced by analysis of cDNA clones from cell line KG-1 and brain. DNA Res. 3: 321-329, 1996. [PubMed: 9039502, related citations] [Full Text]

  5. Seki, N., Hattori, A., Sugano, S., Muramatsu, M., Saito, T. cDNA cloning, expression profile, and genomic structure of human and mouse RNF10/Rnf 10 genes, encoding a novel RING finger protein. J. Hum. Genet. 45: 38-42, 2000. [PubMed: 10697961, related citations] [Full Text]


Creation Date:
Patricia A. Hartz : 9/5/2014
mgross : 09/16/2014
mcolton : 9/5/2014

* 615998

RING FINGER PROTEIN 10; RNF10


Alternative titles; symbols

KIAA0262


HGNC Approved Gene Symbol: RNF10

Cytogenetic location: 12q24.31     Genomic coordinates (GRCh38): 12:120,534,356-120,577,588 (from NCBI)


TEXT

Description

Proteins with RING finger domains, such as RNF10, have roles in development, transcriptional regulation, signal transduction, DNA repair, apoptosis, and oncogenesis (Hoshikawa et al., 2008).


Cloning and Expression

By sequencing clones obtained from a size-fractionated KG-1 immature myeloid cell line cDNA library, Nagase et al. (1996) obtained a partial RNF10 clone, which they designated KIAA0262. Northern blot analysis detected variable expression of 2 RNF10 transcripts in all adult tissues and cell lines examined. Highest expression was detected in heart and skeletal muscle.

By searching an EST database for sequences similar to mouse Rnf10, followed by 5-prime and 3-prime RACE analysis of a fetal brain cDNA library, Seki et al. (2000) cloned human RNF10. The deduced 811-amino acid human protein shares 91% identity with the 804-amino acid mouse protein. Both mouse and human RNF10 have an N-terminal zinc-binding C3HC4-type RING finger domain followed by 3 nuclear localization signals. RT-PCR detected Rnf10 expression in all adult and fetal mouse tissues examined.


Gene Function

Using protein interaction assays, Lin et al. (2005) found that RNF10 interacted with MEOX2 (600535), a homeobox transcription factor that regulates proliferation, differentiation, and migration of vascular smooth muscle cells and cardiomyocytes. Domain analysis revealed that the C-terminal region of RNF10 interacted with a central region of MEOX2 between the histidine/glutamine-rich region and the homeodomain. RNF10 and MEOX2 individually activated a p21(WAF1) (CDKN1A; 116899) reporter in transfected NIH-3T3 cells, and together they showed synergistic activation of p21(WAF1).

Myelin-associated glycoprotein (MAG; 159460) is detected in Schwann cells prior to the onset of myelination, suggesting that MAG initiates myelination. Using a Schwann cell-specific element (SSE) in the promoter region of rat Mag as bait, Hoshikawa et al. (2008) identified an interaction between Mag and Rnf10 in rat Schwann cells. EMSA and chromatin immunoprecipitation assays confirmed the interaction of Rnf10 with the SSE of Mag. Overexpression of Rnf10 in Schwann cells increased Mag promoter activity, and activation of the Mag promoter required the RING finger domain of Rnf10. Knockdown of Rnf10 in cultured Schwann cells decreased Mag expression, increased Schwann cell proliferation, and reduced myelination of neurons in Schwann cell-neuron cocultures. Rnf10 showed lower activation of the Mag reporter when expressed in rat osteosarcoma cells, suggesting that Rnf10 requires a Schwann cell-specific partner for maximal Mag activation.


Gene Structure

Seki et al. (2000) determined that the RNF10 gene contains 17 exons and spans approximately 40 kb.


Mapping

Using radiation hybrid analysis, Nagase et al. (1996) mapped the RNF10 gene to chromosome 12.

Hartz (2014) mapped the RNF10 gene to chromosome 12q24.31 based on an alignment of the RNF10 sequence (GenBank D87451) with the genomic sequence (GRCh38).

By radiation hybrid analysis, Seki et al. (2000) mapped the mouse Rnf10 gene to a region of chromosome 5 that shares homology of synteny with human chromosome 12q23-q24.


REFERENCES

  1. Hartz, P. A. Personal Communication. Baltimore, Md. 9/5/2014.

  2. Hoshikawa, S., Ogata, T., Fujiwara, S., Nakamura, K., Tanaka, S. A novel function of RING finger protein 10 in transcriptional regulation of the myelin-associated glycoprotein gene and myelin formation in Schwann cells. PLoS One 3: e3464, 2008. Note: Electronic Article. [PubMed: 18941509] [Full Text: https://doi.org/10.1371/journal.pone.0003464]

  3. Lin, J., Friesen, M. T., Bocangel, P., Cheung, D., Rawszer, K., Wigle, J. T. Characterization of mesenchyme homeobox 2 (MEOX2) transcription factor binding to RING finger protein 10. Molec. Cell. Biochem. 275: 75-84, 2005. [PubMed: 16335786] [Full Text: https://doi.org/10.1007/s11010-005-0823-3]

  4. Nagase, T., Seki, N., Ishikawa, K., Ohira, M., Kawarabayasi, Y., Ohara, O., Tanaka, A., Kotani, H., Miyajima, N., Nomura, N. Prediction of the coding sequences of unidentified human genes. VI. The coding sequences of 80 new genes (KIAA0201-KIAA0280) deduced by analysis of cDNA clones from cell line KG-1 and brain. DNA Res. 3: 321-329, 1996. [PubMed: 9039502] [Full Text: https://doi.org/10.1093/dnares/3.5.321]

  5. Seki, N., Hattori, A., Sugano, S., Muramatsu, M., Saito, T. cDNA cloning, expression profile, and genomic structure of human and mouse RNF10/Rnf 10 genes, encoding a novel RING finger protein. J. Hum. Genet. 45: 38-42, 2000. [PubMed: 10697961] [Full Text: https://doi.org/10.1007/s100380050007]


Creation Date:
Patricia A. Hartz : 9/5/2014

Edit History:
mgross : 09/16/2014
mcolton : 9/5/2014