Alternative titles; symbols
HGNC Approved Gene Symbol: TOMM5
Cytogenetic location: 9p13.2 Genomic coordinates (GRCh38): 9:37,588,413-37,592,597 (from NCBI)
The preprotein translocase of the mitochondrial outer membrane (TOM) complex requires import receptors, an import channel, and small proteins, such as TOMM5, that regulate complex assembly (Kato and Mihara, 2008).
Using mass spectrometry to identify proteins within the HeLa cell TOM complex, Kato and Mihara (2008) identified TOMM5, which they called TOM5. The deduced 51-amino acid protein has a putative C-terminal transmembrane domain. Epitope-tagged TOM5 colocalized with the mitochondrial marker TIM17 (TIMM17A; 605057) in network structures in HeLa cells. Extraction and protease experiments confirmed that TOM5 associated with the TOM complex.
Using in vitro-synthesized small TOM proteins, Kato and Mihara (2008) found that TOM5 was assembled into an approximately 440-kD complex that included the mitochondrial import channel TOM40 (608061). RNA interference-mediated knockdown of TOM40 significantly reduced the levels of all small TOM proteins, including TOM5, TOM6 (TOMM6; 616168), and TOM7 (TOMM7; 607980). Knockdown of TOM5 or TOM6 had only modest effects on TOM complex stability, whereas knockdown of TOM7 significantly reduced TOM complex stability. Knockdown of the small TOM proteins had only minor effects on protein import and mitochondrial morphology.
Hartz (2015) mapped the TOMM5 gene to chromosome 9p13.2 based on an alignment of the TOMM5 sequence (GenBank BC029423) with the genomic sequence (GRCh38).
Hartz, P. A. Personal Communication. Baltimore, Md. 1/6/2015.
Kato, H., Mihara, K. Identification of Tom5 and Tom6 in the preprotein translocase complex of human mitochondrial outer membrane. Biochem. Biophys. Res. Commun. 369: 958-963, 2008. [PubMed: 18331822] [Full Text: https://doi.org/10.1016/j.bbrc.2008.02.150]