Alternative titles; symbols
HGNC Approved Gene Symbol: DRAIC
Cytogenetic location: 15q23 Genomic coordinates (GRCh38): 15:69,561,720-69,571,440 (from NCBI)
Long noncoding RNAs (lncRNAs), like DRAIC, are regulatory RNAs that contain over 200 nucleotides and show no protein-coding potential (Sakurai et al., 2015).
By database analysis, followed by quantitative RT-PCR, Sakurai et al. (2015) identified DRAIC, which showed higher expression in androgen-dependent human LNCaP prostate cancer cells compared with LNCaP-derived androgen-independent C4-2B cells. DRAIC is a spliced transcript of 1.7 kb that shows no protein-coding potential. Sakurai et al. (2015) also identified 3 splice variants with additional 5-prime exons that were more weakly expressed in LNCaP cells. Semiquantitative RT-PCR showed that DRAIC was expressed mainly in the cytoplasm of LNCaP cells.
By quantitative RT-PCR, Sakurai et al. (2015) found that DRAIC was downregulated by androgen in LNCaP cells. DRAIC was also downregulated in androgen-independent human prostate cancer cell lines. Chromatin immunoprecipitation and PCR analyses confirmed that androgen receptor (AR; 313700) was recruited to the DRAIC promoter region. FOXA1 (602294) and NKX3-1 (602041) were also recruited to the DRAIC promoter in a region that overlapped 3 AR-binding sites. Sakurai et al. (2015) found that AR downregulated DRAIC expression, whereas FOXA1 and NKX3-1 upregulated it. AR and FOXA1 had identical but independent effects on expression of PCAT29 (616273), which lies 20 kb downstream of DRAIC and encodes a lncRNA. Overexpression and inhibitor studies indicated that DRAIC promoted proliferation but inhibited migration and invasion of LNCaP cells, whereas PCAT29 inhibited LNCaP proliferation. Database analysis suggested that expression of DRAIC is a predictor of good prognoses in diverse types of cancer.
Sakurai et al. (2015) determined that the DRAIC gene contains 10 exons and spans approximately 108 kb.
By genomic sequence analysis, Sakurai et al. (2015) mapped the DRAIC gene to chromosome 15q23, 20 kb upstream of the PCAT29 gene.
Sakurai, K., Reon, B. J., Anaya, J., Dutta, A. The lncRNA DRAIC/PCAT29 locus constitutes a tumor-suppressive nexus. Molec. Cancer Res. 13: 828-838, 2015. [PubMed: 25700553] [Full Text: https://doi.org/10.1158/1541-7786.MCR-15-0016-T]