Entry - *616522 - DCN1 DOMAIN-CONTAINING PROTEIN 5; DCUN1D5 - OMIM

 
 
* 616522

DCN1 DOMAIN-CONTAINING PROTEIN 5; DCUN1D5


Alternative titles; symbols

SQUAMOUS CELL CARCINOMA-RELATED ONCOGENE 5; SCCRO5


HGNC Approved Gene Symbol: DCUN1D5

Cytogenetic location: 11q22.3     Genomic coordinates (GRCh38): 11:103,050,686-103,092,160 (from NCBI)


TEXT

▼ Description

DCUN1D5 belongs to a family of enzymes that promote neddylation, the covalent attachment of NEDD8 (603171) to target proteins. Like ubiquitination, neddylation requires an E1 activating enzyme (NAE1 (603385)-UBA3 (UBE1C; 603172)), an E2 conjugating enzyme (UBC12 (UBE2M; 603173) or UBE2F (617700)), and an E3 ligating enzyme. DCUN1D5 is predicted to function as E3 enzyme in the neddylation of cullins (see CUL1, 603134) (Bommelje et al., 2014).


▼ Cloning and Expression

Bommelje et al. (2014) reported that the deduced 237-amino acid DCUN1D5 protein, which they called SCCRO5, contains an N-terminal nuclear localization signal followed by a PONY (potentiation of neddylation) domain.


▼ Gene Function

Using protein pull-down assays, followed by Western blot analysis, Bommelje et al. (2014) found that SCCRO5 interacted with several neddylation components in HeLa cells, including CAND1 (607727), UBC12, CUL1, CUL2 (603135), CUL3 (603136), and ROC1 (RBX1; 603814). SCCRO5 preferentially bound to the NEDD8-UBC12 thioester, rather than to free UBC12, suggesting that it functions as an E3 enzyme in neddylation of cullins. In an in vitro neddylation reaction, SCCRO5 enhanced cullin neddylation in a dose-dependent manner. In transfected mouse NIH-3T3 fibroblasts, Sccro5 increased proliferation, soft agar colony formation, and anchorage-independent growth. Quantitative RT-PCR revealed SCCRO5 overexpression in human cancers that harbored chromosome 11q22 amplification.


▼ Mapping

Bommelje et al. (2014) identified DCUN1D5 in a region of chromosome 11q22 commonly amplified in cancers.

Hartz (2015) mapped the DCUN1D5 gene to chromosome 11q22.3 based on an alignment of the DCUN1D5 sequence (GenBank AK056993) with the genomic sequence (GRCh38).

▼ REFERENCES

  1. Bommelje, C. C., Weeda, V. B., Huang, G., Shah, K., Bains, S., Buss, E., Shaha, M., Goenen, M., Ghossein, R., Ramanathan, S. Y., Singh, B. Oncogenic function of SCCRO5/DCUN1D5 requires its neddylation E3 activity and nuclear localization. Clin. Cancer Res. 20: 372-381, 2014. [PubMed: 24192928, images, related citations] [Full Text]

  2. Hartz, P. A. Personal Communication. Baltimore, Md. 8/13/2015.


Creation Date:
Patricia A. Hartz : 8/13/2015
Edit History:
mgross : 09/27/2017
mgross : 08/18/2015
mcolton : 8/13/2015

* 616522

DCN1 DOMAIN-CONTAINING PROTEIN 5; DCUN1D5


Alternative titles; symbols

SQUAMOUS CELL CARCINOMA-RELATED ONCOGENE 5; SCCRO5


HGNC Approved Gene Symbol: DCUN1D5

Cytogenetic location: 11q22.3     Genomic coordinates (GRCh38): 11:103,050,686-103,092,160 (from NCBI)


TEXT

Description

DCUN1D5 belongs to a family of enzymes that promote neddylation, the covalent attachment of NEDD8 (603171) to target proteins. Like ubiquitination, neddylation requires an E1 activating enzyme (NAE1 (603385)-UBA3 (UBE1C; 603172)), an E2 conjugating enzyme (UBC12 (UBE2M; 603173) or UBE2F (617700)), and an E3 ligating enzyme. DCUN1D5 is predicted to function as E3 enzyme in the neddylation of cullins (see CUL1, 603134) (Bommelje et al., 2014).


Cloning and Expression

Bommelje et al. (2014) reported that the deduced 237-amino acid DCUN1D5 protein, which they called SCCRO5, contains an N-terminal nuclear localization signal followed by a PONY (potentiation of neddylation) domain.


Gene Function

Using protein pull-down assays, followed by Western blot analysis, Bommelje et al. (2014) found that SCCRO5 interacted with several neddylation components in HeLa cells, including CAND1 (607727), UBC12, CUL1, CUL2 (603135), CUL3 (603136), and ROC1 (RBX1; 603814). SCCRO5 preferentially bound to the NEDD8-UBC12 thioester, rather than to free UBC12, suggesting that it functions as an E3 enzyme in neddylation of cullins. In an in vitro neddylation reaction, SCCRO5 enhanced cullin neddylation in a dose-dependent manner. In transfected mouse NIH-3T3 fibroblasts, Sccro5 increased proliferation, soft agar colony formation, and anchorage-independent growth. Quantitative RT-PCR revealed SCCRO5 overexpression in human cancers that harbored chromosome 11q22 amplification.


Mapping

Bommelje et al. (2014) identified DCUN1D5 in a region of chromosome 11q22 commonly amplified in cancers.

Hartz (2015) mapped the DCUN1D5 gene to chromosome 11q22.3 based on an alignment of the DCUN1D5 sequence (GenBank AK056993) with the genomic sequence (GRCh38).

REFERENCES

  1. Bommelje, C. C., Weeda, V. B., Huang, G., Shah, K., Bains, S., Buss, E., Shaha, M., Goenen, M., Ghossein, R., Ramanathan, S. Y., Singh, B. Oncogenic function of SCCRO5/DCUN1D5 requires its neddylation E3 activity and nuclear localization. Clin. Cancer Res. 20: 372-381, 2014. [PubMed: 24192928] [Full Text: https://doi.org/10.1158/1078-0432.CCR-13-1252]

  2. Hartz, P. A. Personal Communication. Baltimore, Md. 8/13/2015.


Creation Date:
Patricia A. Hartz : 8/13/2015

Edit History:
mgross : 09/27/2017
mgross : 08/18/2015
mcolton : 8/13/2015



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OMIM® and Online Mendelian Inheritance in Man® are registered trademarks of the Johns Hopkins University.
Copyright® 1966-2024 Johns Hopkins University.

NOTE: OMIM is intended for use primarily by physicians and other professionals concerned with genetic disorders, by genetics researchers, and by advanced students in science and medicine. While the OMIM database is open to the public, users seeking information about a personal medical or genetic condition are urged to consult with a qualified physician for diagnosis and for answers to personal questions.
OMIM® and Online Mendelian Inheritance in Man® are registered trademarks of the Johns Hopkins University.
Copyright® 1966-2024 Johns Hopkins University.
Printed: April 23, 2024