Alternative titles; symbols
HGNC Approved Gene Symbol: SWI5
Cytogenetic location: 9q34.11 Genomic coordinates (GRCh38): 9:128,275,356-128,288,989 (from NCBI)
SWI5 functions with SFR1 (616527) to promote DNA recombination and repair (Yuan and Chen, 2011).
Akamatsu and Jasin (2010) cloned 2 splice variants of Swi5 from mouse embryonic stem (ES) cells. The transcripts have different first exons, and the deduced 89- and 121-amino acid proteins differ at their N-terminal ends. They are identical in the predicted coiled-coil domain and C-terminal sequence. Immunofluorescence analysis showed colocalization of Swi5 with Sfr1 in the nucleus of mouse ES cells and embryonic fibroblasts.
Yuan and Chen (2011) reported that human SWI5 contains 235 amino acids. Database analysis revealed orthologs from mammals to yeast, all of which share similarity in the coiled-coil domain and the C-terminal domain. RT-PCR analysis detected SWI5 expression in all cell lines tested, and EST database analysis suggested widespread SWI5 expression in human tissues.
Akamatsu and Jasin (2010) found that mouse Sfr1 and Swi5 formed a complex in vivo and in vitro. The Swi5-Sfr1 complex also interacted with Rad51 (179617), a DNA strand-exchange protein that functions during homologous recombination repair, through Swi5. Knockout of either Swi5 or Sfr1 in mouse ES cells reduced expression of the other protein and increased cell sensitivity to agents that cause DNA strand breaks.
Independently, Yuan and Chen (2011) found that human SWI5 and MEI5 interacted in the nucleus and provided mutual stability. Protein pull-down experiments revealed that the SWI5-MEI5 complex also interacted with RAD51. In U2OS cells, the SWI5-MEI5 complex promoted RAD51 foci formation and homologous recombination on damaged DNA following ionizing radiation-induced DNA damage. Knockdown of SWI5 or MEI5 impaired RAD51 foci formation on damaged DNA following ionizing radiation.
Yuan and Chen (2011) stated that the SWI5 gene maps to chromosome 9q34.11.
Akamatsu, Y., Jasin, M. Role for the mammalian Swi5-Sfr1 complex in DNA strand break repair through homologous recombination. PLoS Genet. 6: e1001160, 2010. Note: Electronic Article. [PubMed: 20976249] [Full Text: https://doi.org/10.1371/journal.pgen.1001160]
Hartz, P. A. Personal Communication. Baltimore, Md. 8/17/2015.
Yuan, J., Chen, J. The role of the human SWI5-MEI5 complex in homologous recombination repair. J. Biol. Chem. 286: 9888-9893, 2011. [PubMed: 21252223] [Full Text: https://doi.org/10.1074/jbc.M110.207290]