* 616571

C-TYPE LECTIN DOMAIN FAMILY 18, MEMBER A; CLEC18A


HGNC Approved Gene Symbol: CLEC18A

Cytogenetic location: 16q22.1     Genomic coordinates (GRCh38): 16:69,943,519-69,966,623 (from NCBI)


TEXT

Description

CLEC18A belongs to a large superfamily of C-type lectins that bind polysaccharides and function in a wide range of host-pathogen and cell-cell interactions (Huang et al., 2015).


Cloning and Expression

Huang et al. (2015) reported that the deduced 446-amino acid CLEC18A protein contains an N-terminal signal peptide, followed by an SCF/CAP domain, an EGF (131530) region, an EGF-like region, and a C-type lectin-like domain (CTLD). CLEC18A has 2 potential N-glycosylation sites. CLEC18A is almost identical to CLEC18C (616573), and both share a high degree of similarity with CLEC18B (616572). Real-time PCR detected CLEC18A in most of the cell lines tested and in normal human peripheral blood cells. Western blot analysis of transfected 293T cells detected CLEC18A with an apparent molecular mass of 50 kD, which was reduced to 45 kD upon glycosidase treatment. Flow cytometry and Western blot analysis with antibody that did not differentiate among CLEC18 proteins showed abundant expression in human blood cells, including T cells, B cells, granulocytes, and monocytes, with further upregulation in monocyte-derived dendritic cells (DCs) and macrophages. ELISA also detected CLEC18 in culture medium of human DCs and macrophages and in human sera. Immunohistochemical analysis showed CLEC18 in splenic leukocytes, microglia of brain, hepatocytes in liver, and epithelial cells of gallbladder, fallopian tube, and testis. Immunofluorescence analysis and cell fractionation revealed CLEC18 in endoplasmic reticulum, Golgi, and endosomes in cultured human macrophages.


Gene Function

Huang et al. (2015) found that the isolated CTLD of CLEC18A bound the F3 polysaccharide of Ganoderma lucidum, as well as zymosans and house dust mite. Competition assays with a panel of polysaccharides revealed that CLEC18A bound beta-1,3-linked glucans, beta-1,4-linked galactan, and sulfated fucose polymer. Binding was independent of calcium.


Gene Structure

Huang et al. (2015) reported that the CLEC18A gene contains 13 exons. Exon 1 is noncoding.


Mapping

By genomic sequence analysis, Huang et al. (2015) mapped the CLEC18A gene to chromosome 16q22.1.


REFERENCES

  1. Huang, Y.-L., Pai, F.-S., Tsou, Y.-T., Mon, H.-C., Hsu, T.-L., Wu, C.-Y., Chou, T.-Y., Yang, W.-B., Chen, C.-H., Wong, C.-H., Hsieh, S.-L. Human CLEC18 gene cluster contains C-type lectins with differential glycan-binding specificity. J. Biol. Chem. 290: 21252-21263, 2015. [PubMed: 26170455, images, related citations] [Full Text]


Creation Date:
Patricia A. Hartz : 9/23/2015
alopez : 07/16/2019
mgross : 09/23/2015
mgross : 9/23/2015

* 616571

C-TYPE LECTIN DOMAIN FAMILY 18, MEMBER A; CLEC18A


HGNC Approved Gene Symbol: CLEC18A

Cytogenetic location: 16q22.1     Genomic coordinates (GRCh38): 16:69,943,519-69,966,623 (from NCBI)


TEXT

Description

CLEC18A belongs to a large superfamily of C-type lectins that bind polysaccharides and function in a wide range of host-pathogen and cell-cell interactions (Huang et al., 2015).


Cloning and Expression

Huang et al. (2015) reported that the deduced 446-amino acid CLEC18A protein contains an N-terminal signal peptide, followed by an SCF/CAP domain, an EGF (131530) region, an EGF-like region, and a C-type lectin-like domain (CTLD). CLEC18A has 2 potential N-glycosylation sites. CLEC18A is almost identical to CLEC18C (616573), and both share a high degree of similarity with CLEC18B (616572). Real-time PCR detected CLEC18A in most of the cell lines tested and in normal human peripheral blood cells. Western blot analysis of transfected 293T cells detected CLEC18A with an apparent molecular mass of 50 kD, which was reduced to 45 kD upon glycosidase treatment. Flow cytometry and Western blot analysis with antibody that did not differentiate among CLEC18 proteins showed abundant expression in human blood cells, including T cells, B cells, granulocytes, and monocytes, with further upregulation in monocyte-derived dendritic cells (DCs) and macrophages. ELISA also detected CLEC18 in culture medium of human DCs and macrophages and in human sera. Immunohistochemical analysis showed CLEC18 in splenic leukocytes, microglia of brain, hepatocytes in liver, and epithelial cells of gallbladder, fallopian tube, and testis. Immunofluorescence analysis and cell fractionation revealed CLEC18 in endoplasmic reticulum, Golgi, and endosomes in cultured human macrophages.


Gene Function

Huang et al. (2015) found that the isolated CTLD of CLEC18A bound the F3 polysaccharide of Ganoderma lucidum, as well as zymosans and house dust mite. Competition assays with a panel of polysaccharides revealed that CLEC18A bound beta-1,3-linked glucans, beta-1,4-linked galactan, and sulfated fucose polymer. Binding was independent of calcium.


Gene Structure

Huang et al. (2015) reported that the CLEC18A gene contains 13 exons. Exon 1 is noncoding.


Mapping

By genomic sequence analysis, Huang et al. (2015) mapped the CLEC18A gene to chromosome 16q22.1.


REFERENCES

  1. Huang, Y.-L., Pai, F.-S., Tsou, Y.-T., Mon, H.-C., Hsu, T.-L., Wu, C.-Y., Chou, T.-Y., Yang, W.-B., Chen, C.-H., Wong, C.-H., Hsieh, S.-L. Human CLEC18 gene cluster contains C-type lectins with differential glycan-binding specificity. J. Biol. Chem. 290: 21252-21263, 2015. [PubMed: 26170455] [Full Text: https://doi.org/10.1074/jbc.M115.649814]


Creation Date:
Patricia A. Hartz : 9/23/2015

Edit History:
alopez : 07/16/2019
mgross : 09/23/2015
mgross : 9/23/2015