Entry - *619507 - ZINC FINGER PROTEIN 425; ZNF425 - OMIM

 
 
* 619507

ZINC FINGER PROTEIN 425; ZNF425


HGNC Approved Gene Symbol: ZNF425

Cytogenetic location: 7q36.1     Genomic coordinates (GRCh38): 7:149,102,784-149,126,324 (from NCBI)


TEXT

▼ Description

ZNF425 is a zinc finger protein that functions as a transcriptional repressor in the mitogen-activated protein kinase (MAPK; see 176948) signaling pathway (Wang et al., 2011).


▼ Cloning and Expression

Using RACE, Wang et al. (2011) cloned ZNF425 from a human embryonic heart cDNA library. The deduced 752-amino acid ZNF425 protein has a calculated molecular mass of 88.7 kD. ZNF425 contains a highly conserved N-terminal Kruppel-associated box (KRAB) domain and at least 19 C2H2 zinc finger motifs that extend to the end of the protein. Human ZNF425 is closely related to mouse Znf26 (194537). Northern blot analysis detected a 3.2-kb ZNF425 transcript in 30-day human embryos, but not in 60-day embryos or adult tissues. Immunofluorescence analysis revealed that fluorescence-tagged ZNF425 localized to nuclei of transfected COS-7 cells, with some expression in cytoplasm.


▼ Gene Function

Using a luciferase reporter gene, Wang et al. (2011) showed that ZNF425 functioned as a transcriptional repressor. Overexpression of ZNF425 inhibited serum response element (SRE), AP1 (165160), and SRF (600589) transcriptional activities in COS-7 cells, suggesting that ZNF425 functions in the MAPK signaling pathway as a transcriptional inhibitor. Deletion analysis revealed that the C2H2 zinc finger domain of ZNF425 was the main region responsible for repression.


▼ Gene Structure

Wang et al. (2011) determined that ZNF425 gene contains 4 exons.


▼ Mapping

Gross (2021) mapped the ZNF425 gene to chromosome 7q36.1 based on an alignment of the ZNF425 sequence (GenBank BC125189) with the genomic sequence (GRCh38).

▼ REFERENCES

  1. Gross, M. B. Personal Communication. Baltimore, Md. 8/27/2021.

  2. Wang, Y., Ye, X., Zhou, J., Wan, Y., Xie, H., Deng, Y., Yan, Y., Li, Y., Fan, X., Yuan, W., Mo, X., Wu, X. A novel human KRAB-related zinc finger gene ZNF425 inhibits mitogen-activated protein kinase signaling pathway. BMB Rep. 44: 58-63, 2011. [PubMed: 21266108, related citations] [Full Text]


Contributors:
Matthew B. Gross - updated : 08/27/2021
Creation Date:
Bao Lige : 08/27/2021
Edit History:
mgross : 08/27/2021

* 619507

ZINC FINGER PROTEIN 425; ZNF425


HGNC Approved Gene Symbol: ZNF425

Cytogenetic location: 7q36.1     Genomic coordinates (GRCh38): 7:149,102,784-149,126,324 (from NCBI)


TEXT

Description

ZNF425 is a zinc finger protein that functions as a transcriptional repressor in the mitogen-activated protein kinase (MAPK; see 176948) signaling pathway (Wang et al., 2011).


Cloning and Expression

Using RACE, Wang et al. (2011) cloned ZNF425 from a human embryonic heart cDNA library. The deduced 752-amino acid ZNF425 protein has a calculated molecular mass of 88.7 kD. ZNF425 contains a highly conserved N-terminal Kruppel-associated box (KRAB) domain and at least 19 C2H2 zinc finger motifs that extend to the end of the protein. Human ZNF425 is closely related to mouse Znf26 (194537). Northern blot analysis detected a 3.2-kb ZNF425 transcript in 30-day human embryos, but not in 60-day embryos or adult tissues. Immunofluorescence analysis revealed that fluorescence-tagged ZNF425 localized to nuclei of transfected COS-7 cells, with some expression in cytoplasm.


Gene Function

Using a luciferase reporter gene, Wang et al. (2011) showed that ZNF425 functioned as a transcriptional repressor. Overexpression of ZNF425 inhibited serum response element (SRE), AP1 (165160), and SRF (600589) transcriptional activities in COS-7 cells, suggesting that ZNF425 functions in the MAPK signaling pathway as a transcriptional inhibitor. Deletion analysis revealed that the C2H2 zinc finger domain of ZNF425 was the main region responsible for repression.


Gene Structure

Wang et al. (2011) determined that ZNF425 gene contains 4 exons.


Mapping

Gross (2021) mapped the ZNF425 gene to chromosome 7q36.1 based on an alignment of the ZNF425 sequence (GenBank BC125189) with the genomic sequence (GRCh38).

REFERENCES

  1. Gross, M. B. Personal Communication. Baltimore, Md. 8/27/2021.

  2. Wang, Y., Ye, X., Zhou, J., Wan, Y., Xie, H., Deng, Y., Yan, Y., Li, Y., Fan, X., Yuan, W., Mo, X., Wu, X. A novel human KRAB-related zinc finger gene ZNF425 inhibits mitogen-activated protein kinase signaling pathway. BMB Rep. 44: 58-63, 2011. [PubMed: 21266108] [Full Text: https://doi.org/10.5483/BMBRep.2011.44.1.58]


Contributors:
Matthew B. Gross - updated : 08/27/2021

Creation Date:
Bao Lige : 08/27/2021

Edit History:
mgross : 08/27/2021



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OMIM® and Online Mendelian Inheritance in Man® are registered trademarks of the Johns Hopkins University.
Copyright® 1966-2024 Johns Hopkins University.

NOTE: OMIM is intended for use primarily by physicians and other professionals concerned with genetic disorders, by genetics researchers, and by advanced students in science and medicine. While the OMIM database is open to the public, users seeking information about a personal medical or genetic condition are urged to consult with a qualified physician for diagnosis and for answers to personal questions.
OMIM® and Online Mendelian Inheritance in Man® are registered trademarks of the Johns Hopkins University.
Copyright® 1966-2024 Johns Hopkins University.
Printed: April 25, 2024