Alternative titles; symbols
HGNC Approved Gene Symbol: FAM168B
Cytogenetic location: 2q21.1 Genomic coordinates (GRCh38): 2:131,047,876-131,093,460 (from NCBI)
FAM168B modulates neurogenesis and neurite outgrowth (Mishra et al., 2011).
By bioinformatic analysis, Mishra et al. (2011) showed that FAM168B, which they called MANI, contains 2 potential transmembrane domains, a potential opioid receptor (see 600018)-like motif, an acetylation site, and several phosphorylation sites. Topologically, MANI has a large extracellular loop, with its short N and C termini in the cytoplasm. RT-PCR and immunohistochemical analyses revealed that MANI was expressed in human brain tissue, specifically in myelinated neurons. Analysis with mouse brain showed that Mani was an N-glycoprotein that localized to neuronal cell membranes.
By immunocytochemical analysis, Mishra et al. (2012) showed that Mani localized to the perinuclear area and to the cell membrane of differentiated rat neuronal B104 cells.
Gross (2022) mapped the FAM168B gene to chromosome 2q21.1 based on an alignment of the FAM168B sequence (GenBank BC066347) with the genomic sequence (GRCh38).
Mishra et al. (2011) showed that MANI expression was downregulated in brains of patients with Alzheimer disease (AD; 104300), as well as in brains of an AD mouse model. Ectopic expression of human MANI in catecholaminergic PC12 cells induced alterations in phosphorylation and the cleavage pattern of App (104760). Mani was not expressed in mouse neural stem cells (NSCs), but it was expressed in differentiated neurons and promoted their survival. Mani overexpression promoted NSC differentiation and neurogenesis, and it inhibited neurite outgrowth through Stmn2 (600621) and Stat3 (102582) in catecholaminergic PC12 cells. Furthermore, Mani interacted with Cdc27 (116946) to modulate the cell cycle, thereby regulating survival and differentiation of neurons.
Mishra et al. (2012) showed that Mani inhibited neurite outgrowth, as knockdown of Mani in primary rat cortical neurons had significantly longer neurites than controls. A yeast-2-hybrid screen revealed that Mani interacted with several proteins involved in governing neurite outgrowth.
Gross, M. B. Personal Communication. Baltimore, Md. 10/13/2022.
Mishra, M., Akatsu, H., Heese, K. The novel protein MANI modulates neurogenesis and neurite-cone growth. J. Cell. Molec. Med. 15: 1713-1725, 2011. [PubMed: 20716133] [Full Text: https://doi.org/10.1111/j.1582-4934.2010.01134.x]
Mishra, M., Lee, S., Lin, M. K., Yamashita, T., Heese, K. Characterizing the neurite outgrowth inhibitory effect of Mani. FEBS Lett. 586: 3018-3023, 2012. [PubMed: 22771904] [Full Text: https://doi.org/10.1016/j.febslet.2012.06.043]