Alternative titles; symbols
HGNC Approved Gene Symbol: TMEM158
Cytogenetic location: 3p21.31 Genomic coordinates (GRCh38): 3:45,224,466-45,226,287 (from NCBI)
TMEM158 is a brain-specific cell surface receptor (Hama et al., 2001).
Hama et al. (2001) cloned rat Tmem158, which they called p40Bbp. The predicted rat Tmem158 protein contains 285 amino acids and has a calculated molecular mass of 29 kD. It has 2 to 4 putative transmembrane domains and 3 consensus sites for N-glycosylation. Immunostaining showed that p40Bbp had an apparent molecular mass 40 kD, which was reduced to 30 kD following deglycosylation. Northern blot analysis detected p40Bbp expression in rat brain only, and expression increased after birth. Immunofluorescence assays showed that p40Bbp localized to the plasma membrane of transfected COS7 cells.
Iglesias et al. (2006) noted that TMEM158, which they called RIS1, is considered a pseudogene in the Ensembl database. The authors stated that RIS1 encodes a putative 300-amino acid protein. An imperfect trinucleotide repeat encodes a 14-alanine tract at the C-terminal end of RIS1 and confers high sensitivity to replication errors.
Using transfected COS7 cells, Hama et al. (2001) showed p40Bbp bound to brain injury-derived neurotrophic peptide (BINP), a synthetic 13-mer peptide. Analysis with rat hippocampal neurons revealed that p40Bbp bound to BINP as a brain-specific receptor protein and that binding to BINP prevented cell death.
By analyzing sporadic colorectal tumors, Iglesias et al. (2006) identified RIS1 as a target gene in the mutator pathway of colorectal carcinogenesis.
Iglesias et al. (2006) noted that the TMEM158 gene contains 1 exon and spans 1 kb.
Iglesias et al. (2006) stated that the TMEM158 gene maps to chromosome 3p21.3.
Hama, T., Maruyama, M., Katoh-Semba, R., Takizawa, M., Iwashima, M., Nara, K. Identification and molecular cloning of a novel brain-specific receptor protein that binds to brain injury-derived neurotrophic peptide: possible role for neuronal survival. J. Biol. Chem. 276: 31929-31935, 2001. [PubMed: 11399754] [Full Text: https://doi.org/10.1074/jbc.M100617200]
Iglesias, D., Fernandez-Peralta, A. M., Nejda, N., Daimiel, L., Azcoita, M. M., Oliart, S., Gonzalez-Aguilera, J. J. RIS1, a gene with trinucleotide repeats, is a target in the mutator pathway of colorectal carcinogenesis. Cancer Genet. Cytogenet. 167: 138-144, 2006. [PubMed: 16737913] [Full Text: https://doi.org/10.1016/j.cancergencyto.2005.12.002]