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Induction of read through activity at cricket paralysis virus-internal ribosome entry site premature termination codon UGA assessed as rate constant for octapeptide formation from FKVRQ-tRNAGln at 500 uM preincubated for 30 secs in presence of Typ-tRNA followed by Leu-tRNA and [35S]-Met-tRNA addition by co-sedimentation assay
Assay data:1 Tested
SummaryPubMed Citation
Induction of read through activity at cricket paralysis virus-internal ribosome entry site codon UGG assessed as rate constant for octapeptide formation from FKVRQ-tRNAGln at 500 uM preincubated for 30 secs in presence of Typ-tRNA followed by Leu-tRNA and [35S]-Met-tRNA addition by co-sedimentation assay
Induction of read through activity at cricket paralysis virus-internal ribosome entry site codon UGG assessed as rate constant for octapeptide formation from FKVRQ-tRNAGln at 5 uM preincubated for 30 secs in presence of Typ-tRNA followed by Leu-tRNA and [35S]-Met-tRNA addition by co-sedimentation assay
Induction of read through activity at cricket paralysis virus-internal ribosome entry site premature termination codon UGA assessed as increase in octapeptide formation from FKVRQ-tRNAGln measured after 20 mins in presence of Typ-tRNA, Leu-tRNA and [35S]-Met-tRNA by co-sedimentation assay
Assay data:12 Active, 12 Activity ≤ 1 µM, 15 Tested
SummaryCompounds, ActiveCompounds, activity ≤ 1 µMPubMed Citation
Inhibition of read through activity at cricket paralysis virus-internal ribosome entry site codon UGG assessed as decrease in octapeptide formation from FKVRQ-tRNAGln at 600 uM measured after 20 mins in presence of Typ-tRNA, Leu-tRNA and [35S]-Met-tRNA by co-sedimentation assay
Assay data:1 Active, 1 Tested
SummaryCompounds, ActivePubMed Citation
Inhibition of read through activity at cricket paralysis virus-internal ribosome entry site premature termination codon UGA assessed as decrease in octapeptide formation from FKVRQ-tRNAGln at 600 uM measured after 20 mins in presence of Typ-tRNA, Leu-tRNA and [35S]-Met-tRNA by co-sedimentation assay
Induction of read through activity at cricket paralysis virus-internal ribosome entry site codon UGG assessed as eEF2-induced 80S-Trp-IRES-PRE6 formation from 80S-Trp-IRES-POST5 by FRET assay
Induction of read through activity at cricket paralysis virus-internal ribosome entry site premature termination codon UGA assessed as eEF2-induced 80S-Stop-IRES-PRE6 formation from 80S-Stop-IRES-POST5 at 1 to 10 uM by FRET assay
Induction of read through activity at cricket paralysis virus-internal ribosome entry site codon UGG assessed as increase in octapeptide formation from FKVRQ-tRNAGln at 30 to 600 uM measured after 20 mins in presence of Typ-tRNA, Leu-tRNA and [35S]-Met-tRNA by co-sedimentation assay
Assay data:4 Tested
Induction of read through activity at cricket paralysis virus-internal ribosome entry site codon UGG assessed as increase in octapeptide formation from FKVRQ-tRNAGln at EC50 to 2 times EC50 measured after 20 mins in presence of Typ-tRNA, Leu-tRNA and [35S]-Met-tRNA by co-sedimentation assay
Assay data:17 Tested
Induction of read through activity at cricket paralysis virus-internal ribosome entry site premature termination codon UGA assessed as ratio of plateau octapeptide to FKVRQ-tRNAGln measured after 20 mins in presence of Typ-tRNA, Leu-tRNA and [35S]-Met-tRNA by co-sedimentation assay
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