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Inhibition of recombinant Caulobacter vibrioides N-terminal His6-tagged DHp-catalytic domain DivJ (188 to 585 residues) expressed in Escherichia coli BL21 (DE3) at 800 uM preincubated for 10 mins followed by [gamma-32P]-ATP addition after 15 mins by phosphotransferase dot blot assay
Assay data:1 Active, 1 Tested
SummaryCompounds, ActivePubMed CitationRelated BioAssays by Target
Inhibition of recombinant Caulobacter vibrioides N-terminal His6-tagged DHp-catalytic domain DivJ (188 to 585 residues) expressed in Escherichia coli BL21 (DE3) at 400 uM preincubated for 10 mins followed by [gamma-32P]-ATP addition after 15 mins by phosphotransferase dot blot assay
Assay data:1 Tested
SummaryPubMed CitationRelated BioAssays by Target
Inhibition of recombinant Caulobacter vibrioides N-terminal His6-tagged DHp-catalytic domain DivJ (188 to 585 residues) expressed in Escherichia coli BL21 (DE3) preincubated for 10 mins followed by [gamma-32P]-ATP addition after 15 mins by phosphotransferase dot blot assay
Assay data:10 Tested
Inhibition of recombinant Caulobacter vibrioides N-terminal His6-tagged DHp-catalytic domain DivJ (188 to 585 residues) autophosphorylation expressed in Escherichia coli BL21(DE3) at 500 uM preincubated for 10 mins followed by [gamma-32P]-ATP addition after 15 mins by phosphotransferase dot blot assay relative to control
Assay data:15 Tested
Experimentally measured binding affinity data (Ki) for protein-ligand complexes derived from PDB
Assay data:3598 Active, 421 Activity ≤ 1 nM, 2233 Activity ≤ 1 µM, 3598 Tested
SummaryCompounds, ActiveCompounds, activity ≤ 1 µMPubMed CitationRelated BioAssays by DepositorRelated BioAssays by Target
Experimentally measured binding affinity data derived from PDB
Assay data:3073 Active, 3073 Tested
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