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Binding affinity to mouse 5-F-Trp-SPSB2 assessed as induction of sharpening of Trp207 resonance by measuring increase in peak height at protein to compound molar ratio of 1:5 by 19F-NMR spectroscopy
Assay data:1 Tested
SummaryPubMed CitationRelated BioAssays by Target
Binding affinity to mouse 5-F-Trp-SPSB2 assessed as downward shift of Trp207 residue resonance at protein to compound molar ratio of 1:5 by 19F-NMR spectroscopy
Assay data:4 Active, 5 Tested
SummaryCompounds, ActivePubMed CitationRelated BioAssays by Target
Ratio of Kd for recombinant GST-tagged mouse SPSB2 (12 to 224 residues) expressed in Escherichia coli BL21 (DE3) by ITC method to Kd for recombinant GST-tagged mouse SPSB2 (12 to 224 residues) expressed in Escherichia coli BL21 by SPR analysis
Potency index, ratio of Ac-DINNNNH2 Kd to compound Kd for recombinant GST-tagged mouse SPSB2 (12 to 224 residues) expressed in Escherichia coli BL21 (DE3) by SPR analysis
Binding affinity to recombinant GST-tagged mouse SPSB2 (12 to 224 residues) expressed in Escherichia coli BL21 (DE3) by ITC method
Assay data:5 Active, 5 Activity ≤ 1 µM, 5 Tested
SummaryCompounds, ActiveCompounds, activity ≤ 1 µMPubMed CitationRelated BioAssays by Target
Binding affinity to recombinant GST-tagged mouse SPSB2 (12 to 224 residues) expressed in Escherichia coli BL21 (DE3) assessed as dissociation rate by SPR analysis
Assay data:4 Tested
Binding affinity to recombinant GST-tagged mouse SPSB2 (12 to 224 residues) expressed in Escherichia coli BL21 (DE3) by SPR analysis
Assay data:4 Active, 4 Activity ≤ 1 µM, 4 Tested
Binding affinity to recombinant GST-tagged mouse SPSB2 (12 to 224 residues) expressed in Escherichia coli BL21 (DE3) assessed as association rate by SPR analysis
Genome-wide siRNA screen of genes regulating the Lipopolysaccharide-induced NF-kappaB and TNF-alpha responses in mouse macrophages_Primary screen NFkB readout
Assay data:762 Active, 16821 Tested
SummaryRelated BioAssays by Same Project
Genome-wide siRNA screen of genes regulating the Lipopolysaccharide-induced NF-kappaB and TNF-alpha responses in mouse macrophages_Primary screen TNF readout
Assay data:1106 Active, 16821 Tested
Displacement of full-length of iNOS from SPSB2 in LPS/IFNgamma-stimulated mouse BMDM cell lysate at 10 uM after 2 hrs by Western blotting analysis
Assay data:1 Active, 1 Tested
A screen to identify the genes that modify the response of osteosarcoma to doxorubicin in mouse - Primary Screen
Assay data:788 Active, 16789 Tested
SummaryPubMed Citation
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