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Inhibition of human C-terminal domain DNMT3b catalytic domain (568 to 853 residues) expressed in Escherichia coli BL21 (DE3) pLysS cells assessed as inhibition of methylated dI-dC formation using poly dI-dC and [Me-3H]SAM as substrate at 100 uM incubated for 45 mins in presence of DNMT3L by liquid scintillation counting analysis
Assay data:1 Tested
SummaryPubMed CitationRelated BioAssays by Target
Inhibition of human C-terminal domain DNMT3b catalytic domain (568 to 853 residues) expressed in Escherichia coli BL21 (DE3) pLysS cells assessed as inhibition of methylated dI-dC formation using poly dI-dC and [Me-3H]SAM as substrate incubated for 45 mins in presence of DNMT3L by liquid scintillation counting analysis
Assay data:5 Active, 2 Activity ≤ 1 µM, 7 Tested
SummaryCompounds, ActiveCompounds, activity ≤ 1 µMPubMed CitationRelated BioAssays by Target
Inhibition of DNMT3B/3L (unknown origin) at 50 uM relative to control
Inhibition of human full length N-terminal GST tagged recombinant DNMT3b (2 to 853 residues) expressed in Sf9 insect cells at 50 uM using Lambda DNA as substrate in presence of [3H]-SAM by hotspot assay relative to control
Inhibition of DNMT3B/3L (unknown origin) at 10 uM
Inhibition of human full length N-terminal GST tagged recombinant DNMT3b (2 to 853 residues) expressed in Sf9 insect cells at 10 uM using Lambda DNA as substrate in presence of [3H]-SAM by hotspot assay
Inhibition of DNMT3B (unknown origin) at 1 to 10 uM
Assay data:2 Tested
Binding affinity to DNMT3b PWWP domain (unknown origin) assessed as aggregation temperature at 400 uM by heating at 25 to 85 degC by differential static light scattering method
Inhibition of human DNMT3B in human KG-1 cells expressing cmv-firefly luciferase assessed as increase in luminescence at 5 uM incubated for 24 hrs by luciferase reporter gene assay relative to control
Assay data:3 Tested
Inhibition of human DNMT3B in human KG-1 cells expressing cmv-firefly luciferase assessed as increase in luminescence incubated for 24 hrs by luciferase reporter gene assay relative to control
Assay data:4 Tested
Inhibition of human DNMT3B expressed in Escherichia coli Rosetta-gamiTM 2(DE3) pLysS competent cells using biotinylated 6-FAM double strand DNA comprising unique CpG site [3H]-SAM at 1 uM incubated for 1 hrs by fluorescence based assay
Inhibition of human DNMT3B in human KG-1 cells expressing cmv-firefly luciferase assessed as increase in luminescence upto 10 uM incubated for 24 hrs by luciferase reporter gene assay relative to control
Assay data:7 Tested
Inhibition of human DNMT3B expressed in Escherichia coli Rosetta-gamiTM 2(DE3) pLysS competent cells using biotinylated 6-FAM double strand DNA comprising unique CpG site [3H]-SAM incubated for 1 hrs by fluorescence based assay
Assay data:10 Active, 14 Tested
SummaryCompounds, ActivePubMed CitationRelated BioAssays by Target
Inhibition of human recombinant DNMT3B expressed in baculovirus infected insect cells by DRONE assay
Assay data:2 Active, 1 Activity ≤ 1 µM, 2 Tested
Inhibition of human recombinant DNMT3B expressed in baculovirus infected insect cells using CpG site containing internally quenched hairpin oligonucleotide DNA substrate and SAM incubated for 30 mins by kinetic fluorogenic assay
Assay data:4 Active, 1 Activity ≤ 1 µM, 21 Tested
Inhibition of human DNMT3B using [3H]-SAM
Assay data:1 Active, 1 Activity ≤ 1 µM, 1 Tested
Inhibition of DNMT3B/3L (unknown origin) at 5 uM
Inhibition of DNMT3B/3L (unknown origin) using poly(2'-deoxyinosinic-2'-deoxycytidylic acid) as substrate after 1 hr by scintillation proximity assay
Inhibition of N-terminal GST-tagged recombinant full length human DNMT3b (2 to 853 residues) using bacteriophage lambda DNA as substrate preincubated for 20 mins followed by [3H]-SAM and measured after 60 mins by scintillation counting
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