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Inhibition of COX-1 in mouse J774 cells assessed as inhibition of PGE2 production using arachidonic acid as substrate at 10 uM preincubated for 2 hrs followed by incubation with arachidonic acid for 30 mins by ELISA
Assay data:1 Active, 1 Tested
SummaryCompounds, ActivePubMed CitationRelated BioAssays by Target
Inhibition of COX-1 in mouse J774 cells assessed as inhibition of PGE2 production using arachidonic acid as substrate at 0.1 to 10 uM preincubated for 2 hrs followed by incubation with arachidonic acid for 30 mins by ELISA
Assay data:1 Tested
SummaryPubMed CitationRelated BioAssays by Target
Inhibition of COX1 in mouse J774 cells using arachidonic acid as substrate preincubated for 15 mins followed substrate addition and assessed as inhibition of PGE2 production after 30 mins by enzyme immuno-assay (EIA)
Assay data:1 Active, 19 Tested
Inhibition of COX-1 in mouse J774 cells assessed as inhibition of PEG2 production using arachidonic acid as substrate pretreated for 15 mins followed by substrate addition measured after 30 mins by radioimmuno assay
Assay data:8 Tested
Inhibition of COX-1 in LPS-induced mouse peritoneal macrophages
Assay data:1 Active, 1 Activity ≤ 1 µM, 1 Tested
SummaryCompounds, ActiveCompounds, activity ≤ 1 µMPubMed CitationRelated BioAssays by Target
Inhibition of COX1 in mouse BV2 cells assessed as reduction in LPS-stimulated PGE2 release by measuring PGE2 level at 1 uM preincubated for 1 hr followed by LPS addition measured after 48 hrs by immunoassay (Rvb = 0.7555 +/- 0.032 ng/ml)
Inhibition of COX1 in mouse BV2 cells assessed as reduction in LPS-stimulated PGE2 release by measuring PGE2 level at 0.5 uM preincubated for 1 hr followed by LPS addition measured after 48 hrs by immunoassay (Rvb = 0.7555 +/- 0.032 ng/ml)
Assay data:2 Tested
Inhibition of COX1 in mouse BV2 cells assessed as reduction in LPS-stimulated PGE2 release by measuring PGE2 level at 10 uM preincubated for 1 hr followed by LPS addition measured after 48 hrs by immunoassay (Rvb = 0.7555 +/- 0.032 ng/ml)
Inhibition of COX-1 in mouse RAW264.7 cells assessed as reduction in PGE2 production at 10 uM after 24 hrs by enzyme immunoassay
Inhibition of COX1 in C57BL/6 mouse peritoneal macrophages assessed as reduction in PGE2 formation pre-incubated for 2 hrs followed by arachidonic acid addition and measured after 10 mins by radio-immuno assay or enzyme-immunoassay
Assay data:124 Tested
SummaryRelated BioAssays by Target
Inhibition of COX1 in mouse J774 cells using arachidonic acid as substrate assessed as decrease in PGE2 production at 10 uM preincubated for 15 mins followed by substrate addition measured after 30 mins by radioimmunoassay relative to control
Assay data:52 Tested
Inhibition of COX-1 in mouse J774 cells assessed as reduction in PGE2 level using arachidonic acid as substrate preincubated for 15 mins followed by incubation with arachidonic acid for 30 mins by radio immunoassay
Genome-wide siRNA screen of genes regulating the Lipopolysaccharide-induced NF-kappaB and TNF-alpha responses in mouse macrophages_Primary screen NFkB readout
Assay data:762 Active, 16821 Tested
SummaryRelated BioAssays by Same Project
Genome-wide siRNA screen of genes regulating the Lipopolysaccharide-induced NF-kappaB and TNF-alpha responses in mouse macrophages_Primary screen TNF readout
Assay data:1106 Active, 16821 Tested
Inhibition of calcimycin-induced COX-1 in peritoneal macrophage of C57BL/J6 mouse assessed as 6-Keto prostaglandin F1alpha formation preincubated for 1 hr followed by calcimycin challenge by radioimmunoassay
Inhibition of COX-1 in mouse J774 cells using arachidonic acid as substrate assessed as inhibition of prostaglandin E2 production at 10 uM preincubated for 15 mins followed by substrate addition measured after 30 mins by RIA
Assay data:20 Tested
Inhibition of COX-1 in mouse J774 cells using arachidonic acid as substrate assessed as inhibition of prostaglandin E2 production preincubated for 15 mins followed by substrate addition measured after 30 mins by RIA
A screen to identify the genes that modify the response of osteosarcoma to doxorubicin in mouse - Primary Screen
Assay data:788 Active, 16789 Tested
SummaryPubMed Citation
Inhibition of COX1 in mouse J774 cells using arachidonic acid as substrate assessed as inhibition of PGE2 production incubated for 15 mins prior to substrate addition measured after 30 mins by radioimmunoassay
Assay data:1 Active, 13 Tested
Inhibition of arachidonic acid-induced COX1 activity in mouse J774 cells assessed as decrease in PGE2 levels incubated for 15 mins prior to arachidonic acid-challenge measured after 30 mins by RIA
Assay data:2 Active, 14 Tested
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