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Protac activity at CRBN/UBE2T in human BxPC-3 cells assessed as UBE2T degradation incubated for 16 hrs by proteomic analysis
Assay data:1 Tested
SummaryPubMed CitationRelated BioAssays by Target
RNAi screen for vemurafenib enhancer genes in BRAFV600 melanoma - Primary Screen
Assay data:790 Active, 18119 Tested
Summary
Inhibition of full length N-terminal 6xHis-Smt3 tagged human Ube2T expressed in Escherichia coli BL21(DE3) assessed as FANCD2 ubiquitination at 2.5 mM after 15 mins in presence of fluorescently-labeled Ub/recombinant human E1/FANCL in 0.05% Tween20 containing buffer by fluorescence assay
Assay data:3 Active, 3 Tested
SummaryCompounds, ActivePubMed CitationRelated BioAssays by Target
Binding affinity to full length N-terminal 6xHis-Smt3 tagged human Ube2T expressed in Escherichia coli BL21(DE3) by ITC
Binding affinity to [1H-15N]-labeled N-terminal 6xHis-Smt3 tagged human Ube2T (1 to 154 residues) expressed in Escherichia coli BL21(DE3) by chemical shift perturbation assay
Assay data:6 Tested
Binding affinity to full length recombinant N-terminal 6xHis-Smt3 tagged human Ube2T P73K mutant expressed in Escherichia coli BL21(DE3) by ITC
Inhibition of full length N-terminal 6xHis-Smt3 tagged human Ube2T expressed in Escherichia coli BL21(DE3) assessed as FANCD2 ubiquitination after 15 mins in presence of fluorescently-labeled Ub/recombinant human E1/ATP by fluorescence assay
Assay data:3 Tested
Reversible binding affinity to human 15N-labelled C-terminal flexible tail lacking Ube2T (1 to 154 residues) at 100 to 500 uM measured after overnight dialysis by NMR spectrocopy
Assay data:1 Active, 1 Tested
Binding affinity to human 15N-labelled C-terminal flexible tail lacking Ube2T (1 to 154 residues) (unknown origin) at 500 uM by NMR spectrocopy
Binding affinity to N-terminally 6xHis-Smt3 tagged human Ube2T C86K/K91R/K95R mutant expressed in Escherichia coli by DSF method
Binding affinity to biotin labeled Ube2T C86K/K91R/K95R mutant (unknown origin) assessed as association and dissociation response at 2 uM by biolayer interferometry analysis
Inhibition of N-terminally 6xHis-Smt3 tagged human Ube2T C86K/K91R/K95R mutant expressed in Escherichia coli charging with ubiquitin after 10 mins in presence of E1 and ATP by coomassie staining based SDS PAGE analysis
Binding affinity to N-terminally 6xHis-Smt3 tagged human Ube2T C86K/K91R/K95R mutant expressed in Escherichia coli in presence of EDTA by ITC method
Binding affinity to N-terminally 6xHis-Smt3 tagged Ub fused human Ube2T C86K/K91R/K95R mutant expressed in Escherichia coli at 1.5 mM by ITC method
Binding affinity to N-terminally 6xHis-Smt3 tagged FANCL RING fused human Ube2T C86K/K91R/K95R mutant expressed in Escherichia coli by ITC method
Assay data:5 Tested
Binding affinity to N-terminally 6xHis-Smt3 tagged human Ube2T C86K/K91R/K95R mutant expressed in Escherichia coli by ITC method
Assay data:2 Tested
Genome-wide siRNA screen of genes regulating the Lipopolysaccharide-induced TNF-alpha response in human macrophages_Primary screen TNF readout
Assay data:2853 Active, 18113 Tested
SummaryRelated BioAssays by Same Project
A screen to identify synthetic lethal interactions with E-cadherin in an MCF10A isogenic cell line
Assay data:2442 Active, 18052 Tested
SummaryPubMed Citation
A screen to identify genes that regulate lymphatic endothelial cell migration - Primary Screen
Assay data:1034 Active, 18120 Tested
SummaryRelated BioAssays by Depositor
Automated high-throughput/high-content microscopy assay for measuring IRIF kinetics
Assay data:13 Active, 41 Tested
SummaryPubMed CitationRelated BioAssays by Depositor
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