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Binding affinity to human partial length TRIM33 (D882 to A1087 residues) expressed in bacterial expression system by BROMOscan assay
Assay data:4 Tested
SummaryRelated BioAssays by Target
Binding affinity to N-terminal His6-TEV-tagged human TRIM33B transfected in Escherichia coli BL21 (DE3) assessed as thermal stabilization by measuring change in melting temperature at 100 uM by SYPRO orange dye based differential scanning fluorimetry analysis
Assay data:1 Tested
SummaryPubMed CitationRelated BioAssays by Target
Binding affinity to TRIM33A (unknown origin) transfected in Escherichia coli BL21 (DE3) assessed as thermal stabilization by measuring change in melting temperature at 100 uM by SYPRO orange dye based differential scanning fluorimetry analysis
Binding affinity to partial length human TRIM33 (D882 to A1087 residues) expressed in bacterial system at 1 uM by BROMOscan method relative to control
Binding affinity to human TRIM33 bromodomain assessed as residual activity at 300 nM by BROMOscan method relative to control
TRIM33(PHD,Bromo.) DiscoverX BROMOscan
TRIM33 (TIF1gamma) (PHD & BD) DiscoverX BROMOscan
TRIM33 (PHD,BD) DiscoverX BROMOscan
Inhibition of human TRIM33 BD2 (D882 to A1087 residues) at 10 uM by DiscoverX assay relative to control
Thermal Shift Assay. Domain start/stop: D882-P1073
Assay data:2 Tested
Assay data:3 Tested
Alphascreen assay. Domain start/stop: D882-P1073
Assay data:5 Tested
Inhibition of human partial length TRIM33 (D882 to A1087 residues) expressed in bacterial expression system by BROMOscan assay
Binding affinity to human partial length TRIM33 phd bromodomain (D882 to A1087 residues) expressed in bacterial expression system by BROMOscan assay
Assay data:1 Active, 1 Activity ≤ 1 nM, 1 Activity ≤ 1 µM, 1 Tested
SummaryCompounds, ActiveCompounds, activity ≤ 1 µMPubMed CitationRelated BioAssays by Target
Inhibition of recombinant TRIM33 PHD bromodomain (unknown origin) using biotinylated H3 peptide (H3K9me3K18ac) by AlphaScreen assay
Assay data:35 Active, 16 Activity ≤ 1 µM, 110 Tested
SummaryCompounds, ActiveCompounds, activity ≤ 1 µMRelated BioAssays by Target
Binding affinity to human partial length TRIM33 (D882 to A1087 residues) expressed in Escherichia coli BL21 cells assessed as residual binding level at 1 uM measured after 1 hr by quantitative PCR based BROMOscan assay relative to control
Binding affinity to human partial length TRIM33 (D882 to A1087 residues) expressed in bacterial expression system at 1 uM by BROMOscan assay
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