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Inhibition of human DNA polymerase beta at 100 uM incubated for 60 mins by Microbeta counter analysis
Assay data:1 Tested
SummaryPubMed CitationRelated BioAssays by Target
Substrate activity at human DNA polymerase beta incorporated with HIV reverese transcriptase using 5'-Cy3-CGTTG GTCCT GAAGG AGGAT AGG1T-3' as primer and 3'-GCAAC CAGGA CTTCC TCCTA TCCAA AGACA-5' as template incubated at 37 degree C for 60 mins and at 80 degreeC for 3 mins by primer extension assay
Substrate activity at human DNA polymerase beta using 5'-Cy3-CGTTG GTCCT GAAGG AGGAT AGG1T-3' as primer and 3'-GCAAC CAGGA CTTCC TCCTA TCCAA AGACA-5' as template incubated at 37 degree C for 60 mins and at 80 degreeC for 3 mins by primer extension assay
Assay data:1 Active, 2 Tested
SummaryCompounds, ActivePubMed CitationRelated BioAssays by Target
DNA Pol β PEX Assay from Article 10.1021/cb4007562: "Expanding the Scope of Human DNA Polymerase and Inhibitors."
Assay data:25 Tested
SummaryPubMed CitationRelated BioAssays by DepositorRelated BioAssays by Target
Substrate activity at human DNA polymerase beta incubated at 37 degC for 60 mins and at 80 degC for 7 mins by primer extension assay
Assay data:1 Active, 3 Tested
Substrate activity at human DNA polymerase beta assessed as increase in primer extension incubated at 37 degC for 60 mins and at 80 degC for 3 mins in presence of fluorescent-lebelled dATP, dCTP and dGTP by PAGE analysis
Assay data:3 Tested
Binding affinity to wild type human DNA polymerase beta assessed as inhibition of dRP excision in presence of 32P-end labelled duplex DNA at 25 uM by SDS PAGE analysis
Assay data:3 Active, 3 Tested
Binding affinity to human DNA polymerase beta with methyl-lysine modification at 72 assessed as inhibition of dRP excision in presence of 32P-end labelled duplex DNA at 25 uM by SDS PAGE
Binding affinity to human DNA polymerase beta with homo-lysine modification at 72 assessed as inhibition of dRP excision in presence of 32P-end labelled duplex DNA at 25 uM by SDS PAGE
Inhibition of human DNA polymerase beta at 100 uM using calf DNA as substrate by microbeta scintillation counting method relative to control
Assay data:6 Tested
Inhibition of human DNA polymerase-beta by fluorescence/radiometric assay
Inhibition of human DNA polymerase beta using 5-end radiolabeled 24nt to 48nt DNA as primer template after 5 mins in presence of dCTP/dGTP/dTTP/dATP by PAGE analysis
Inhibition of human DNA polymerase-beta assessed as reduction in [3H]dTTP incorporation in to activated calf thymus DNA substrate at 100 uM by scintillation counting method relative to control
Assay data:8 Tested
RNAi screen for vemurafenib enhancer genes in BRAFV600 melanoma - Primary Screen
Assay data:790 Active, 18119 Tested
Summary
Inhibition of human DNA polymerase beta
Inhibition of human DNA polymerase beta using 5' end radiolabeled 24nt DNA/48nt DNA as primer/template after 5 mins by PAGE analysis
Inhibition of human DNA polymerase-beta
Inhibition of recombinant human DNA polymerase beta preincubated with enzyme followed by addition of dATP/dGTP/TTP as substrate in presence of [gamma-33P]TTP by phosphorimaging assay
Inhibition of human DNA polymerase-beta expressed in wild-type 92TAg cells assessed as increase in MMS toxicity at 1 to 60 uM incubated for 2 hrs prior to MMS challenge for 2 hrs followed by post wash compound addition measured after 24 hrs by MTT assay
Assay data:2 Tested
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