Warning: The NCBI web site requires JavaScript to function. more...
An official website of the United States government
The .gov means it's official. Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you're on a federal government site.
The site is secure. The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.
Inhibition of recombinant human Asparagine endopeptidase preincubated for 5 mins followed by Z-Ala-Ala-Asn-AMC substrate addition measured after 2 hrs by microplate reader
Assay data:6 Active, 5 Activity ≤ 1 µM, 10 Tested
SummaryCompounds, ActiveCompounds, activity ≤ 1 µMPubMed CitationRelated BioAssays by Target
Inhibition of recombinant human Asparagine endopeptidase at 10 uM preincubated for 5 mins followed by Z-Ala-Ala-Asn-AMC substrate addition measured after 2 hrs by microplate reader relative to control
Assay data:10 Tested
SummaryPubMed CitationRelated BioAssays by Target
Inhibition Assay from US Patent US9345789: "Specific inhibitors and active site probes for legumain"
Assay data:3 Active, 3 Activity ≤ 1 µM, 5 Tested
SummaryCompounds, ActiveCompounds, activity ≤ 1 µMRelated BioAssays by Target
Inhibition of Legumain (unknown origin) at 10 uM
Assay data:3 Tested
Inhibition of recombinant human legumain using Z-Ala-Ala-Asn-AMC as substrate measured for every 2 mins for 60 mins by fluorescence-based microplate reader analysis
Assay data:26 Active, 3 Activity ≤ 1 nM, 26 Activity ≤ 1 µM, 27 Tested
Inhibition of legumain (unknown origin) at 10 uM
Assay data:1 Active, 1 Tested
SummaryCompounds, ActivePubMed CitationRelated BioAssays by Target
RNAi screen for vemurafenib enhancer genes in BRAFV600 melanoma - Primary Screen
Assay data:790 Active, 18119 Tested
Summary
Inhibition of AEP in human MDA-MB-231 cells assessed as reduction in MMP2 cleavage by gelatin zymography assay
Assay data:1 Tested
Inhibition of AEP (unknown origin) assessed as reduction in MMP2 cleavage by gelatin zymography assay
Inhibition of AEP in human MDA-MB-435 cells
Inhibition of AEP in human MDA-MB-231 cells
Inhibition of AEP (unknown origin) using Cbz-Ala-Ala-Asn-AMC as substrate after 15 mins by fluorescence assay
Assay data:4 Active, 4 Activity ≤ 1 µM, 4 Tested
Inhibition of AEP (unknown origin) assessed as residual activity at 1 uM using Cbz-Ala-Ala-Asn-AMC as substrate after 15 mins by fluorescence assay relative to control
Assay data:26 Tested
Competitive inhibition of AEP (unknown origin) assessed as ratio of Kinact to Ki using varying levels of Z-AAN-AMC substrate after 10 mins by Lineweaver-Burk plot analysis
Competitive inhibition of AEP (unknown origin) using varying levels of Z-AAN-AMC substrate after 10 mins by Lineweaver-Burk plot analysis
Assay data:1 Active, 1 Activity ≤ 1 µM, 1 Tested
Inhibition of recombinant human legumain using Z-Ala-Ala-Asn-MCA as substrate preincubated with protein followed by substrate addition measured every 2 mins for 60 mins by microplate reader analysis
Assay data:25 Active, 9 Activity ≤ 1 µM, 36 Tested
Inhibition of legumain in human MCF7 cells using Z-Ala-Ala-Asn-MCA substrate at 30 uM incubated for 48 hrs by microplate reader analysis
Inhibition of legumain in human MCF7 cells using Z-Ala-Ala-Asn-MCA substrate at 30 uM incubated for 24 hrs by microplate reader analysis
Assay data:28 Tested
Inhibition of recombinant human legumain using Z-Ala-Ala-Asn-MCA substrate measured every 2 mins for 60 mins by microplate reader analysis
Assay data:25 Active, 25 Activity ≤ 1 µM, 29 Tested
Genome-wide siRNA screen of genes regulating the Lipopolysaccharide-induced TNF-alpha response in human macrophages_Primary screen TNF readout
Assay data:2853 Active, 18113 Tested
SummaryRelated BioAssays by Same Project
Filters: Manage Filters
Your browsing activity is empty.
Activity recording is turned off.
Turn recording back on