Warning: The NCBI web site requires JavaScript to function. more...
An official website of the United States government
The .gov means it's official. Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you're on a federal government site.
The site is secure. The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.
Selectivity interaction (Protein methyltransferase panel (activity assay)) EUB0000211b NSD1
Assay data:1 Tested
SummaryRelated BioAssays by Target
Inhibition of human NSD1 at 10 uM by radioisotope-based filter binding assay
SummaryPubMed CitationRelated BioAssays by Target
Inhibition of N-terminal His-tagged NSD1 (1538 to 2696 residues) (unknown origin) expressed in baculovirus expression system at 1 uM using 3H-SAM as a substrate relative to control
Inhibition of human recombinant N-terminal his-tagged NSD1 (1538 to 2696 residues) expressed in Sf9 insect cells at 50 uM by scintillation/filter assay relative to control
Inhibition of human recombinant N-terminal his-tagged NSD1 (1538 to 2696 residues) expressed in Sf9 insect cells at 10 uM by scintillation/filter assay
Inhibition of NSD1 (unknown origin) at 1 to 10 uM
Assay data:2 Tested
HMT Assay from Article 10.1038/nchembio.2304: "An allosteric PRC2 inhibitor targeting the H3K27me3 binding pocket of EED."
Assay data:1 Active, 1 Activity ≤ 1 µM, 1 Tested
SummaryCompounds, ActiveCompounds, activity ≤ 1 µMPubMed CitationRelated BioAssays by DepositorRelated BioAssays by Target
Inhibition of NSD1 (unknown origin) at 10 uM using SAM as substrate measured up to 960 mins by AlphaScreen microplate reader assay relative to control
Inhibition of human NSD1-SET using histone H3.1 as substrate incubated for 1 hr by Western blot analysis
Inhibition of NSD1 (unknown origin) using SAM as substrate preincubated for 10 mins followed by substrate addition measured after 4 hrs by AlphaLISA method
Inhibition of N-terminal His-tagged recombinant human NSD1 (1538 to 2696 residues) expressed in baculovirus infected insect cells using nucleosome as substrate at expressed in 10 uM by radiometric HotSpot assay relative to control
Inhibition of NSD1 (unknown origin) at 100 uM
Inhibition of NSD1 (unknown origin) using biotin-labeled histone substrate assessed as residual activity at 50 uM in presence of [3H-Me]SAM by scintillation proximity assay relative to control
Assay data:24 Tested
Inhibition of human NSD1 up to 50 uM using chicken nucleosome as substrate and in presence of [3H]-SAM incubated for 1 hr by filter-based assay
Inhibition of NSD1 (unknown origin) at 10 uM using [3H-SAM] and nucleosome as substrate incubated for 1 hr by scintillation proximity assay
RNAi screen for vemurafenib enhancer genes in BRAFV600 melanoma - Primary Screen
Assay data:790 Active, 18119 Tested
Summary
Inhibition of NSD1 (unknown origin) preincubated for 15 mins followed by [3H]-SAM addition measured after 4 hrs by liquid scintillation counting
Inhibition of NSD1 (unknown origin) at 50 and 100 uM preincubated for 15 mins followed by substrate and [3H]-SAM addition after 120 mins by MicroBeta counting
Inhibition of NSD1 (unknown origin) at 50 uM using biotin-labeled peptide as substrate and [3H]-SAM measured after 1 hr by scintillation proximity assay
Inhibition of NSD1 (unknown origin) at 1 to 50 uM by TCA precipitation assay
Filters: Manage Filters
Your browsing activity is empty.
Activity recording is turned off.
Turn recording back on