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Binding affinity to 4-5 mM pyridoxal-5'-phosphate assessed as formation of PLP-aldimine by ESI-MS analysis
Assay data:1 Active, 1 Tested
SummaryCompounds, ActivePubMed Citation
Binding affinity to pyridoxal-5'-phosphate assessed as formation of PLP-aldimine by NMR spectroscopy
Binding affinity to pyridoxal-5'-phosphate assessed as PLP-amino acid Schiff base interaction at 5 mM after 1 hr by UV/vis spectroscopy
Binding affinity to pyridoxal-5'-phosphate assessed as formation of PLP-aldoxime at 5 mM after 1 hr by UV/vis spectroscopy
Assay data:4 Active, 9 Tested
Inhibition of rat SDH expressed in Escherichia coli BL21-RIL assessed as formation of pyruvate at 5 mM in phosphate buffer of pH 8 treated for 15 to 20 mins before addition of substrate by HPLC analysis in presence of 10 uM PLP
Assay data:1 Active, 2 Tested
SummaryCompounds, ActivePubMed CitationRelated BioAssays by Target
Inhibition of human recombinant serine racemase assessed as formation of D-serine at 5 mM in phosphate buffer of pH 8 treated for 15 to 20 mins before addition of substrate by HPLC analysis in presence of 10 uM PLP
Inhibition of mouse recombinant serine racemase expressed in Escherichia coli MC1061 assessed as formation of D-serine at 5 mM in phosphate buffer of pH 8 treated for 15 to 20 mins before addition of substrate by HPLC analysis in presence of 10 uM PLP
Assay data:3 Active, 4 Tested
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