Warning: The NCBI web site requires JavaScript to function. more...
An official website of the United States government
The .gov means it's official. Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you're on a federal government site.
The site is secure. The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.
CYP46A1 Enzyme Assay from US Patent US20230312522: "CYP46A1 INHIBITORS AND METHODS OF USE THEREOF"
Assay data:48 Active, 51 Activity ≤ 1 µM, 51 Tested
SummaryCompounds, ActiveCompounds, activity ≤ 1 µMRelated BioAssays by Target
Inhibition of CYP46A1 (unknown origin)
Assay data:3 Active, 3 Activity ≤ 1 µM, 3 Tested
SummaryCompounds, ActiveCompounds, activity ≤ 1 µMPubMed CitationRelated BioAssays by Target
Inhibition of CH24H expressing human 293-F cells for 15 minutes using [14C] cholesterol as substrate and measured after 5 hrs by trypan blue staining based thin-layer chromatography
Assay data:23 Active, 22 Activity ≤ 1 µM, 23 Tested
Inhibitory Assay from US Patent US9963443: "Radiolabeled compounds"
Assay data:13 Active, 13 Activity ≤ 1 µM, 13 Tested
Binding affinity to full length human CYP46A1 expressed in Escherichia coli DH5alpha as cholesterol-24 hydroxylation using cholesterol as substrate incubated for 30 mins in presence of NADPH cytochrome P450 oxidoreductase by gas chromatography-mass spectrometry
Assay data:1 Active, 1 Tested
SummaryCompounds, ActivePubMed CitationRelated BioAssays by Target
Inhibition of CYP46A1 (unknown origin) at 1 uM relative to control
Assay data:1 Tested
SummaryPubMed CitationRelated BioAssays by Target
Binding affinity to substrate bound recombinant human C-terminal 4His-tagged CYP46A1 delta(2 to 50) mutant expressed in Escherichia coli assessed as Koff rate of cholesterol by stopped-flow spectrophotometric method
Assay data:2 Tested
Binding affinity to substrate bound recombinant human C-terminal 4His-tagged CYP46A1 delta(2 to 50) mutant expressed in Escherichia coli assessed as Kon rate of cholesterol by stopped-flow spectrophotometric method
Binding affinity to recombinant human C-terminal 4His-tagged CYP46A1 delta(2 to 50) mutant expressed in Escherichia coli in absence of substrate at 24 degC by stopped-flow spectrophotometric method
Assay data:2 Active, 2 Tested
Binding affinity to recombinant human C-terminal 4His-tagged CYP46A1 delta(2 to 50) mutant expressed in Escherichia coli at 2 uM in presence of substrate by UV-spectrophotometric method
Binding affinity to recombinant human C-terminal 4His-tagged CYP46A1 delta(2 to 50) mutant expressed in Escherichia coli at 50 uM in presence of substrate by UV-spectrophotometric method
Binding affinity to recombinant human C-terminal 4His-tagged CYP46A1 delta(2 to 50) mutant expressed in Escherichia coli at 15 to 30 uM in absence of substrate by UV-spectrophotometric method
Assay data:1 Active, 2 Tested
Activation of recombinant human C-terminal 4His-tagged CYP46A1 delta(2 to 50) mutant expressed in Escherichia coli at 20 uM using cholesterol as substrate measured after 30 mins in presence of cytochrome P450 oxidoreductase and L-Glu by gas chromatography-mass spectrometry relative to control
Assay data:9 Tested
Binding affinity to recombinant human C-terminal 4His-tagged CYP46A1 delta(2 to 50) mutant expressed in Escherichia coli in presence of substrate by UV-spectrophotometric method
Assay data:1 Active, 7 Tested
Binding affinity to recombinant human C-terminal 4His-tagged CYP46A1 delta(2 to 50) mutant expressed in Escherichia coli in absence of substrate by UV-spectrophotometric method
Assay data:9 Active, 1 Activity ≤ 1 µM, 10 Tested
Inhibition of recombinant human C-terminal 4His-tagged CYP46A1 delta(2 to 50) mutant expressed in Escherichia coli at up to 100 uM using cholesterol as substrate measured after 30 mins in presence of cytochrome P450 oxidoreductase by gas chromatography-mass spectrometry
Inhibition of recombinant human C-terminal 4His-tagged CYP46A1 delta(2 to 50) mutant expressed in Escherichia coli at 60 to 100 uM using cholesterol as substrate measured after 30 mins in presence of cytochrome P450 oxidoreductase by gas chromatography-mass spectrometry
Activation of recombinant human C-terminal 4His-tagged CYP46A1 delta(2 to 50) mutant expressed in Escherichia coli at 20 uM using cholesterol as substrate measured after 30 mins in presence of cytochrome P450 oxidoreductase by gas chromatography-mass spectrometry relative to control
Activation of recombinant human C-terminal 4His-tagged CYP46A1 delta(2 to 50) mutant expressed in Escherichia coli using cholesterol as substrate measured after 30 mins in presence of cytochrome P450 oxidoreductase by gas chromatography-mass spectrometry
Assay data:8 Tested
Inhibition of human CH24H expressed in Freestyle-293 cells assessed as reduction in 24HC production at 1 uM incubated for 5 hrs using [14C]cholesterol as substrate relative to control
Assay data:102 Tested
SummaryRelated BioAssays by Target
Filters: Manage Filters
Your browsing activity is empty.
Activity recording is turned off.
Turn recording back on