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Binding affinity to IL-15 (unknown origin)
Assay data:1 Tested
Summary
Binding affinity to recombinant human IL-15 assessed as dissociation constant by SPR analysis
Assay data:6 Active, 7 Tested
Inhibition of IL-15-induced STAT3 phosphorylation in human NK92 cells incubated for 30 mins followed by IL-15 stimulation and measured after 1 hr by alphascreen assay
Assay data:7 Active, 4 Activity ≤ 1 µM, 25 Tested
Inhibition of IL-15 in mouse 32Dbeta cells assessed as decrease in cell growth in presence of IL2 incubated for 30 mins followed by IL2 addition and measured after 2.5 days by alamar blue assay
Assay data:6 Active, 25 Tested
Inhibition of IL-15 in mouse 32Dbeta cells assessed as decrease in cell growth in presence of RLI incubated for 30 mins followed by RLI addition and measured after 2.5 days by alamar blue assay
Assay data:8 Active, 25 Tested
Inhibition of IL-15 (unknown origin)/IL-15Ralpha interaction in human PBMC cells assessed as reduction of IL-17 synthesis preincubated for 30 mins followed by IL-15 addition measured after 48 hrs by ELISA
Assay data:12 Active, 20 Tested
Inhibition of IL-15 (unknown origin)/IL-15Ralpha interaction in human PBMC cells assessed as reduction of TNF-alpha synthesis preincubated for 30 mins followed by IL-15 addition measured after 48 hrs by ELISA
Binding affinity to recombinant human IL-15 by SPR assay
Assay data:17 Tested
Inhibition of IL15R/IL15 interaction in human NK92 cells assessed as reduction of IL15-induced STAT5 phosphorylation preincubated for 30 mins with IL15 followed by cell addition measured after 1 hr by AlphaScreen assay
Inhibition of human IL-15Ralpha-sushi domain/human IL-15-induced mouse 32D-beta cell proliferation preincubated for 30 mins with protein followed by cell addition measured after 2.5 days by Alamar blue reduction assay
Assay data:27 Active, 3 Activity ≤ 1 µM, 51 Tested
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