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Induction of p53 stabilization in human HCT-116 cells assessed as fold increase by Western blot analysis
Assay data:1 Tested
SummaryRelated BioAssays by Target
Inhibition of MDM2/ P53 (unknown origin) interaction
Assay data:1 Active, 1 Activity ≤ 1 nM, 1 Activity ≤ 1 µM, 1 Tested
Antagonist activity at MDM2/ P53 (unknown origin) interaction
Assay data:1 Active, 1 Activity ≤ 1 µM, 1 Tested
Antagonist activity at human N terminal MDM2 (25 to 108 residues)/ N terminal His 6 tagged human P53 (1 to 312 residues)
Inhibition of HDM2-p53 (unknown origin) protein-protein interaction
Assay data:1 Active, 3 Tested
Inhibition of p53 transcriptional activity in human HCT-116 cells assessed as inhibition of protein binding to p21 DNA promoter region at 20 uM incubated for 24 hrs by ChIP analysis
Assay data:3 Active, 3 Tested
Inhibition of p53 DNA binding activity in human HCT-116 cells assessed as reduction in DNA-protein complex formation at 20 uM incubated for 24 hrs by electrophoretic mobility shift analysis
Inhibition of MDM2/p53 (unknown origin) interaction at 10 uM relative to control
Assay data:2 Tested
Inhibition of human DMX-p53 protein-protein interaction by ITC analysis
Assay data:8 Active, 2 Activity ≤ 1 nM, 10 Activity ≤ 1 µM, 10 Tested
Inhibition of human DM2-p53 protein-protein interaction by fluorescence anisotropy analysis
Assay data:7 Active, 3 Activity ≤ 1 nM, 10 Activity ≤ 1 µM, 10 Tested
Inhibition of MDM4 assessed as dissociation constant
Inhibition of human MDM4 by SRP assay
Inhibition of human MDM4 by TR-FRET assay
Assay data:3 Active, 2 Activity ≤ 1 µM, 3 Tested
Inhibition of human MDM4/p53 interaction by TR-FRET assay
Assay data:2 Active, 1 Activity ≤ 1 µM, 2 Tested
Inhibition of human MDM4/p53 interaction by FP assay
Assay data:1 Active, 1 Tested
Inhibition of human MDM4/p53 interaction by immuno enzymatic assay
Inhibition of human MDM2/p53 interaction by immuno enzymatic assay
Inhibition of human his-tagged MDM4 assessed as dissociation constant by ITC assay
Inhibition of human MDM4
Inhibition of human MDM4/p53 interaction
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