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Chemical stability assessed as hydrolysis-associated hydroxylamine formation at 0.5 mM after 1 hr in phosphate buffer of pH 7.4
Assay data:3 Active, 3 Tested
SummaryCompounds, ActivePubMed Citation
Chemical stability assessed as hydrolysis-associated hydroxylamine formation at 0.5 mM after 1 hr in phosphate buffer of pH 8
Binding affinity to pyridoxal-5'-phosphate assessed as formation of PLP-aldoxime by ESI-MS analysis
Assay data:3 Active, 7 Tested
Binding affinity to pyridoxal-5'-phosphate assessed as compound remaining after 12 days by NMR spectroscopy
Assay data:1 Tested
SummaryPubMed Citation
Binding affinity to pyridoxal-5'-phosphate assessed as formation of syn- and anti-PLP-aldoxime by NMR spectroscopy
Assay data:4 Active, 7 Tested
Binding affinity to pyridoxal-5'-phosphate assessed as formation of PLP-aldoxime at 5 mM after 1 hr by UV/vis spectroscopy
Assay data:4 Active, 9 Tested
Inhibition of broad-range bacterial transaminase assessed as formation of L-glutamic acid at 5 mM in phosphate buffer of pH 8 treated for 15 to 20 mins before addition of substrate by HPLC analysis in presence of 10 uM PLP
Assay data:2 Tested
Inhibition of Bacillus stearothermophilus alanine racemase assessed as formation of L-alanine at 5 mM in phosphate buffer of pH 8 treated for 15 to 20 mins before addition of substrate by HPLC analysis in presence of 10 uM PLP
SummaryPubMed CitationRelated BioAssays by Target
Inhibition of rat SDH expressed in Escherichia coli BL21-RIL assessed as formation of pyruvate at 5 mM in phosphate buffer of pH 8 treated for 15 to 20 mins before addition of substrate by HPLC analysis in presence of 10 uM PLP
Assay data:1 Active, 2 Tested
SummaryCompounds, ActivePubMed CitationRelated BioAssays by Target
Inhibition of human recombinant serine racemase assessed as formation of D-serine at 5 mM in phosphate buffer of pH 8 treated for 15 to 20 mins before addition of substrate by HPLC analysis in presence of 10 uM PLP
Inhibition of mouse recombinant serine racemase expressed in Escherichia coli MC1061 assessed as formation of D-serine at 5 mM in phosphate buffer of pH 8 treated for 15 to 20 mins before addition of substrate by HPLC analysis in presence of 10 uM PLP
Assay data:3 Active, 4 Tested
Inhibition of mouse recombinant serine racemase expressed in Escherichia coli MC1061 assessed as formation of D-serine at 5 mM after 30 mins by HPLC analysis
Assay data:11 Tested
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